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１９９３年４～５月．采用实验现场结合组织化学技术，观察了水淹对钉螺春季繁殖器螺卵组织化学的影响。结果表明，对照组螺卵卵胚内的ＳＤＨ、ＬＤＨ及ＣＣｏ等与细胞能量代谢有关的酶呈强阳性反应．实验组螺卵卵胚内的ＳＤＨ．ＣＣｏ，水淹第１０ｄ时降为的阳性反应（＋），３０ｄ时消失，ＬＤＨ含量在水淹在２０ｄ时未见变化，３０ｄ时降为阳性反应（＋＋），４０ｄ时消失。水淹３０ｄ的螺卵石蜡包理切片，作糖原（ＰＡＳ）反应，由强阳性变为弱阳性（＋）．综合上述结果，分析了水淹影响螺卵发育的机制。
April to May 1993. Using the experimental site combined with histochemical techniques, the effect of flooding on the morphological characteristics of snail eggs in the spring snail was observed. The results showed that the control group of egg oocyte embryos SDH, LDH and CCo and other cellular energy metabolism-related enzymes showed a strong positive reaction. Experimental group of spiral eggs in the embryo of SDH. (+) Disappeared on the 10th day after flooding, and disappeared on the 30th day. The content of LDH did not change when the flooding was on the 20th day, but decreased to positive on the 30th day (++), disappeared on the 40th day. Spiraled 30 days and spiked with paraffin wax for glycogen (PAS) reaction, and changed from strong positive to weak positive (+). Based on the above results, the mechanism of flooding on the development of Spiro eggs was analyzed.