论文部分内容阅读
从理论上推导出核酸、蛋白质的正电荷数随pH的变化关系式:Y=sum from i=1 to ∞(([H~+]n_i)/([H~+]+K_(bi))),负电荷数随pH的变化关系式:X=sum from i=1 to 5((K_(ai)m_i)/([H~+]+K_(ai)))、净电荷数随pH变化关系式以及等电点计算关系式,并从关系式中导出核苷酸、碱基、中性氨基酸、酸性氨基酸、碱性氨基酸的等电点关系式,表明理论推导是正确、可靠的。用表面活性剂沉淀肌酸激酶、不同pH的溶菌酶电泳方法验证了计算结果的可靠性。同时,计算了人DNA、蚕DNA、β-乳球蛋白带电荷数随pH的变化规律,并估计了计算误差。
The relationship between the positive charges of nucleic acids and proteins with pH is deduced theoretically: Y = sum from i = 1 to ∞ (([H ~ +] n_i / (H ~ +] + K_bi) ). The relationship between the negative charge and the pH is as follows: X = sum from i = 1 to 5 ((K_ (ai) m_i) / ([H~ +] + K_ (ai) Relationship and isoelectric point calculation of the relationship, and derived from the relationship of nucleotides, bases, neutral amino acids, acidic amino acids, basic amino acid isoelectric point relationship, indicating that the theoretical derivation is correct and reliable. The surfactant was used to precipitate creatine kinase, and the lysozyme electrophoresis with different pH was used to verify the reliability of the calculation results. At the same time, the variation law of the charged number of human DNA, silkworm DNA and β-lactoglobulin with pH was calculated and the calculation error was estimated.