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目的分析血管紧张素转换酶(ACE)基因多态性与原发性高血压(EH)的关系。方法选择煤矿井下接尘工人为研究对象(EH组和对照组各206人),进行职业流行病学调查,并收集研究对象空腹静脉血5 ml,酚氯仿法提取基因组DNA,采用聚合酶链反应(PCR)方法检测ACE基因多态性。比较两组间的基因型、等位基因频率及倒班分布的差异。结果 EH组和对照组倒班作业工人所占的比例分别为42.23%和29.61%,组间分布比较差异有统计学意义(χ2=7.13,P<0.05),EH发生的危险增加了1.74倍。ACE基因II、ID、DD基因型和等位基因I、D的分布,差异无统计学意义(P>0.05),提示ACE基因多态性与EH无直接关系;但倒班与I和D等位基因联合作用的OR值分别为1.86(OR95%CI:1.32～2.63)和1.72(OR95%CI:1.09～2.71),相乘模型交互作用产生的OReg值为0.79,提示倒班与ACE基因I/D等位基因之间存在负交互作用(似然比检验P<0.05)。结论倒班可能是EH的危险因素且可能与ACE基因插入/缺失序列等位基因存在交互作用。
Objective To analyze the relationship between angiotensin converting enzyme (ACE) gene polymorphism and essential hypertension (EH). Methods We selected mine workers exposed to dust in coal mine (206 in EH group and control group) for occupational epidemiological investigation. We collected 5 ml of fasting venous blood samples from patients and extracted genomic DNA with phenol-chloroform method. Polymerase chain reaction (PCR) method to detect ACE gene polymorphism. Differences in genotype, allele frequency, and shift distribution between the two groups were compared. Results The proportion of shift workers in EH group and control group were 42.23% and 29.61%, respectively. There was significant difference between the two groups (χ2 = 7.13, P <0.05), and the risk of EH increased 1.74 times. There was no significant difference in the distribution of ACE gene II, ID, DD genotypes and alleles I and D (P> 0.05), which suggested that there was no direct relationship between ACE gene polymorphism and EH The odds ratio of interaction between genes was 1.86 (OR 95% CI: 1.32-2.63) and 1.72 (OR 95% CI: 1.09-2.71), respectively. There was a negative interaction between the alleles (likelihood ratio test P <0.05). Conclusions Shift may be a risk factor for EH and may interact with ACE gene insertion / deletion sequence alleles.