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目的建立中低温低流量的SD大鼠模型,分析其表型和神经损伤机制。方法 60只SD大鼠随机均分为手术组和假手术组。大鼠肛温降至(25.0±0.5)℃时,手术组夹闭双侧颈总动脉2h后重新放开,假手术组不夹闭颈总动脉。采用脑部血流仪测定缺血-再灌注期间大鼠脑血流量及内环境相关参数的变化,HE染色和TUNEL法观察大鼠神经细胞在缺血-再灌注后不同时间点的变化,Western blot检测凋亡蛋白的表达。结果手术组夹闭颈总动脉后,脑血流率下降至15%左右,血气、血压等指标随体温变化而变化。与假手术组相比,手术组相关神经组织出现损伤,TUNEL阳性细胞与凋亡相关蛋白的表达增加(P<0.01或P<0.05)。结论 SD大鼠中低温低流量的神经系统改变类似于主动脉夹层术中的神经系统缺血-再灌注的变化过程,神经损伤程度与神经细胞的凋亡水平密切相关。
Objective To establish a hypothermia and low flow SD rat model and analyze its phenotype and neural damage mechanism. Methods Sixty SD rats were randomly divided into surgery group and sham operation group. When the rectal temperature of the rats dropped to (25.0 ± 0.5) ℃, the bilateral common carotid artery was occluded in the operation group for 2 hours and then reopened. The common carotid artery was not clamped in the sham operation group. The changes of cerebral blood flow and the related parameters in the rat brain during ischemia-reperfusion were measured by using the cerebral blood flow meter. The changes of neuronal cells at different time points after ischemia-reperfusion were observed by HE staining and TUNEL. blot detection of apoptosis protein expression. Results After the common carotid artery was closed in the operation group, the cerebral blood flow rate dropped to about 15%. The indexes of blood gas and blood pressure varied with the change of body temperature. Compared with the sham-operated group, the expression of TUNEL-positive cells and apoptosis-related proteins increased (P <0.01 or P <0.05). Conclusion The changes of nervous system in middle and low temperature and low fluxes of SD rats are similar to the changes of nervous system ischemia-reperfusion during aortic dissection. The degree of nerve injury is closely related to the apoptosis of nerve cells.