论文部分内容阅读
利用JenniferA.CouchandPaulJ.Fritz的改良方法提取葡萄嫩叶染色体DNA,并进行RFLP分析。结果表明,该方法提取的DNA均未降解;以Sepharose2B层析柱纯化后可进行RFLP分析;核糖体rDNA的PVvc一A片段制备的探针与HindⅢ酶组合,使所有供试样品都显示多态性。
Using JenniferA. CouchandPaulJ. Fritz improved method to extract young leaves of chromosomal DNA, and RFLP analysis. The results showed that all the DNA extracted by this method did not degrade. The purified DNA was purified by Sepharose 2B column and analyzed by RFLP. The probe prepared by PVvc-A fragment of ribosomal rDNA was combined with HindⅢ enzyme to make all the samples show polymorphism Sex.