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单细胞凝胶电泳是一种新近发展起来的快速、敏感地检测单个哺乳动物细胞ＤＮＡ断裂的技术。本文用单细胞凝胶电泳法检测了双歧杆菌及其表面分子———脂磷壁酸、细胞壁肽聚糖对Ｎ－甲基－Ｎ－硝基－Ｎ′－亚硝基胍（ＭＮＮＧ）致小鼠肠粘膜细胞ＤＮＡ损伤的抑制作用。结果双歧杆菌活菌、死菌、脂磷壁酸、细胞壁肽聚糖、双歧杆菌培养乏液在小鼠胃肠反复作用一段时间后，均具有抑制ＤＮＡ损伤的作用，以活菌、死菌作用最强，活菌作用强于死菌，脂磷壁酸和肽聚糖作用次之，二者间无显著性差异，培养乏液也有轻微作用。双歧杆菌及其表面分子的这种抑制ＤＮＡ损伤作用机理，可能是通过结合ＭＮＮＧ，并提高免疫监视功能清除ＭＮＮＧ结合物及ＤＮＡ损伤的细胞。
Single cell gel electrophoresis is a newly developed technique for rapid and sensitive detection of DNA fragmentation in a single mammalian cell. In this paper, single cell gel electrophoresis was used to detect the effects of lipoteichoic acid and cell wall peptidoglycan on N-methyl-N-nitro-N’-nitrosoguanidine (MNNG) Inhibition of intestinal mucosal DNA damage in mice. Results Bifidobacterium live bacteria, dead bacteria, lipoteichoic acid, cell wall peptidoglycan, bifidobacterium culture fluid in mice gastrointestinal repeated role after a period of time, have a role in inhibiting DNA damage to live bacteria, dead The strongest effect of bacteria, live bacteria stronger than dead bacteria, followed by the role of lipoteichoic acid and peptidoglycan, there was no significant difference between the two, lack of liquid culture also have a minor role. This mechanism of DNA damage inhibition by Bifidobacterium and its surface molecules may be due to the binding of MNNG and the enhancement of immune surveillance functions to clear MNNG conjugates and DNA-damaged cells.