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目的：为了研究ＳＬＥ患者ＰＢＭＣ分泌ＩＬ３的能力。方法：采用ＭＴＴ比色方法，用ＩＬ３依赖株（ＴＦ１）分别测定了１５例活动期、１５例缓解期ＳＬＥ患者和正常对照者ＰＢＭＣ培养上清中ＩＬ３的活性水平。结果：ＳＬＥ患者ＰＢＭＣ自发分泌ＩＬ３的活性水平显著高于正常人（Ｐ＜０００１），活动期和缓解期患者之间无显著性差异（Ｐ＞００５），经ＰＨＡ刺激后ＳＬＥ患者和正常人ＰＢＭＣ分泌ＩＬ３的活性水平均显著增加（Ｐ＜０００１），在ＳＬＥ病人中，以伴发显著血小板减少的５例患者ＰＢＭＣ分泌ＩＬ３的活性水平最低。结论：ＩＬ３可能参与ＳＬＥ的致病过程，且和ＳＬＥ患者血小板减少有关。
Objective: In order to study the ability of SLE patients PBMC secretion of IL 3. Methods: MTT colorimetric method was used IL 3-dependent strains (TF1) were measured in 15 active, 15 remission SLE patients and normal control PBMC culture supernatant IL 3 activity levels. Results: The levels of spontaneous IL-3 secreted by peripheral blood mononuclear cells in SLE patients were significantly higher than those in normal controls (P <0001). There was no significant difference between active and remission patients (P> 005) SLE patients and normal PBMC secretion of IL 3 activity levels were significantly increased (P <0001), SLE patients with concomitant significant thrombocytopenia in 5 patients PBMC secretion of IL 3 activity of the lowest level. Conclusion: IL 3 may be involved in the pathogenesis of SLE, and SLE patients with thrombocytopenia.