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目的研究苯那普利对糖尿病大鼠胰岛细胞功能的影响。方法采用长期高脂饮食加小剂量链脲菌素(STZ,30mg/kg)腹腔注射建立2型糖尿病大鼠模型,以苯那普利10mg·kg-1.d-1进行干预,2个月后行口服葡萄糖耐量试验(OGTT)检测胰岛细胞功能,试剂盒检测胰腺组织丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,HE染色和免疫组化对胰腺形态学及十二指肠同源框蛋白-1(PDX-1)进行观察。结果DM组大鼠葡萄糖刺激的胰岛素分泌明显延迟,MDA含量(7.10±0.21)nmol/mg较NC组(3.25±0.12)nmol/mg显著升高(P<0.01),SOD活性(38.9±2.1)U/mg较NC组(51.01±1.47)U/mg显著降低(P<0.01),免疫组化胰岛素染色阳性面积(12.8±2.2)%及PDX-1平均光密度0.240±0.051显著低于NC组[(42.6±2.7)%,(0.648±0.087)nmol/mg](P<0.01),苯那普利干预后胰岛素释放曲线基本接近正常形态,MDA含量(5.87±0.19)nmol/mg较DM组显著降低(P<0.01),SOD活性(45.3±1.7)较DM组显著升高(P<0.01),免疫组化胰岛素染色阳性面积(18.8±2.7)%及PDX-1平均光密度0.40±0.044较DM组显著增高(P<0.01)。结论苯那普利可以减轻糖尿病大鼠氧化应激反应,并上调PDX-1的表达,改善胰岛细胞功能。
Objective To study the effects of benazepril on the function of islet cells in diabetic rats. Methods A rat model of type 2 diabetes mellitus was established by intraperitoneal injection of long-term high-fat diet plus low-dose streptozotocin (STZ, 30 mg / kg), with benazepril 10 mg · kg-1.d-1 for 2 months The function of islet cells was detected by oral glucose tolerance test (OGTT), the content of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) in pancreatic tissue were detected by kit. The morphological changes of pancreas and twelve The bowel homeobox protein-1 (PDX-1) was observed. Results Compared with NC group (3.25 ± 0.12) nmol / mg, MDA content (7.10 ± 0.21) nmol / mg significantly increased (P <0.01) and SOD activity (38.9 ± 2.1) U / mg was significantly lower than NC group (51.01 ± 1.47) U / mg (P <0.01), the positive area of immunohistochemical insulin staining (12.8 ± 2.2)% and PDX-1 average optical density of 0.240 ± 0.051 were significantly lower than NC group (42.6 ± 2.7)%, (0.648 ± 0.087) nmol / mg], respectively (P <0.01). The insulin release profile of benazepril-treated group was close to normal. (P <0.01), SOD activity (45.3 ± 1.7) was significantly higher than DM group (P <0.01), immunohistochemical positive area of insulin staining (18.8 ± 2.7)% and PDX-1 average optical density of 0.40 ± 0.044 Which was significantly higher than DM group (P <0.01). Conclusion benazepril can reduce the oxidative stress in diabetic rats, and up-regulate the expression of PDX-1 and improve the function of islet cells.