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目的了解湖南地区即食食品中食源性致病菌的污染状况和耐药性,为预防和控制食源性疾病提供科学依据。方法从湖南地区的农贸市场、超市和餐饮单位采集即食食品(凉拌菜和熟肉制品),依据GB 4789-2010和GB/T4789-2008方法,对样品进行沙门菌等4种食源性致病菌检测,分离菌株使用微量肉汤稀释法进行药敏试验。结果 991份样品共检出食源性致病菌92株,检出率为9.28%,其中检出金黄色葡萄球菌68株,检出率为6.86%,检出沙门菌和单增李斯特菌各12株,检出率为1.21%,未检出大肠埃希菌O157。药敏结果显示金黄色葡萄球菌和沙门菌均具有一定的耐药性,其中金黄色葡萄球菌对苯唑西林的耐药率高达61.76%,而且多重耐药明显。结论湖南地区即食食品存在一定的食源性致病菌污染,应加大卫生监督力度,防止食源性疾病的暴发流行;同时应控制动物饲料中抗生素的添加使用,加强抗生素的管理和耐药性监测。
Objective To understand the contamination status and drug resistance of food-borne pathogens in ready-to-eat foods in Hunan Province and provide a scientific basis for the prevention and control of food-borne diseases. Methods Ready-to-eat foods (salad and cooked meat products) were collected from farmer’s markets, supermarkets and catering establishments in Hunan Province. According to the methods of GB 4789-2010 and GB / T4789-2008, four food-borne pathogens Detection of bacteria, isolated strains using micro broth dilution method for susceptibility testing. Results A total of 92 strains of food-borne pathogens were detected in 991 samples, with a detection rate of 9.28%. 68 strains of Staphylococcus aureus were detected with a detection rate of 6.86%. Salmonella and Listeria monocytogenes Each 12 strains were detected with a detection rate of 1.21%. Escherichia coli O157 was not detected. Susceptibility results show that both Staphylococcus aureus and Salmonella have a certain degree of resistance, of which Staphylococcus aureus resistance rate to oxacillin is as high as 61.76%, and the multi-drug resistance is obvious. Conclusions There is some food-borne pathogens contamination in ready-to-eat foods in Hunan Province. Health supervision should be strengthened to prevent outbreaks of food-borne diseases. At the same time, the use of antibiotics in animal feed should be controlled to strengthen the management of antibiotics and resistance Sexual monitoring.