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目的 :探讨电磁脉冲 (EMP)辐射对小鼠睾丸间质细胞结构和功能的影响。 方法 :6～ 8周龄二级昆明雄性小鼠 1 1 4只 ,随机分为辐射组 (1 0 8只 )和对照组 (6只 )。辐射组小鼠分别接受 8× 1 0 3、2× 1 0 4 、6× 1 0 4 V/m 3种不同场强EMP 5次重复全身辐射 ,并于辐射后 6h、1d、3d、7d、1 4d和 2 8d应用光镜、电镜观察睾丸间质细胞组织学及超微结构的改变 ,放射免疫法检测小鼠血清睾酮 (T)、黄体生成素 (LH)及雌二醇 (E2 )的浓度。 结果 :3种不同场强EMP辐射后 2 8d内小鼠睾丸间质细胞主要病变表现为细胞水肿和空泡变性 ,胞质线粒体肿胀、数目减少 ,内质网扩张 ,脂滴增多 ,大多数脂滴着色变浅 ,部分或完全排空 ;血清T与对照组相比较均呈不同程度的降低 ,分别在辐射后 6h～ 1 4d、6h～ 7d、1～ 2 8d时显著降低 (P <0 .0 5或P <0 .0 1 ) ,而LH和E2均未发生明显改变。 结论 :睾丸间质细胞是EMP辐射敏感的靶细胞之一 ,EMP辐射可引起小鼠睾丸间质细胞结构和功能的损伤 ,既有早期影响 ,又表现为持续效应 ,可能影响性功能和生精过程
Objective: To investigate the effect of electromagnetic pulse (EMP) radiation on the structure and function of mouse testicular stromal cells. Methods: A total of 114 male Kunming mice aged 6-8 weeks were randomly divided into radiation group (180) and control group (6). The irradiated mice received EMP 5 times repeated whole-body radiation at 8 × 10 3, 2 × 10 4, 6 × 10 4 V / m, respectively. After 6h, 1d, 3d, 7d, The histological and ultrastructural changes of Leydig cells were observed with light and electron microscopy on day 14 and 28, and the levels of serum testosterone (T), luteinizing hormone (LH) and estradiol (E2) were detected by radioimmunoassay concentration. RESULTS: Within 28 days after EMP irradiation, the main pathological changes of testicular stromal cells in mice were cytoplasmic edema and vacuolar degeneration, swelling of cytoplasmic mitochondria, decrease in number, expansion of endoplasmic reticulum, increase of lipid droplets, most of lipid The color of the drop was lighter, partially or completely emptying. The serum T decreased to some extent compared with the control group, which were significantly decreased at 6h ~ 14d, 6h ~ 7d and 1 ~ 28d after radiation (P <0. 0 5 or P <0 01), while neither LH nor E2 significantly changed. Conclusion: Leydig cells are one of the EMP-sensitive target cells. EMP radiation can cause damage to the structure and function of testicular stromal cells in mice, both early and sustained effects may affect sexual function and spermatogenesis process