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目的建立快速测定牛奶中恩诺沙星的化学发光酶免疫检测方法。方法采用竞争法,即将牛奶样品中的恩诺沙星与标记有碱性磷酸酶的恩诺沙星同时与限量的特异性固相恩诺沙星抗体进行竞争结合反应,通过分离未结合的标记抗原,测定标记抗原与抗体复合物化学发光强度,经相应的数学函数计算出待测抗原的含量。根据这一基本原理,利用金刚烷类体系作为化学发光底物,快速地测定牛奶中恩诺沙星残留量。结果检出限可达239.9 pg/ml,检测范围为350～1 000 pg/ml,批内与批间相对标准偏差均小于15%。结论本方法在抗生素恩诺沙星残留检测及监控等领域有很好的应用前景。
Objective To establish a chemiluminescent enzyme immunoassay for rapid determination of enrofloxacin in milk. Methods By competition method, enrofloxacin in milk samples was incubated with enrofloxacin labeled with alkaline phosphatase for competitive binding reaction with limited amount of specific solid phase enrofloxacin antibody, and the unbound conjugate Antigen, measuring the chemiluminescence intensity of the labeled antigen and the antibody complex, and calculating the content of the tested antigen through the corresponding mathematical function. Based on this basic principle, the enrofloxacin residues in milk were rapidly determined using the adamantane system as a chemiluminescent substrate. Results The detection limit was 239.9 pg / ml. The detection range was 350-1 000 pg / ml. The relative standard deviations (RSDs) within and between batches were all less than 15%. Conclusion This method has good application prospects in the field of antibiotic enrofloxacin residue detection and monitoring.