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为建立一种简单快速、高效制备人参皂苷20(S)-Rg3、20(R)-Rg3、Rg5、Rk1的方法。利用大孔吸附树脂和硅胶柱层析对样品进行预处理,通过反相高效制备液相色谱,从红参的甲醇提取物中快速分离得到目标产物人参皂苷20(S)-Rg3、20(R)-Rg3、Rg5、Rk1,经检测,4种化合物的纯度均>98%。色谱柱为Polaris C18(250 mm×4.6 mm,10μm),以V(乙腈)∶V(水)=46∶54为流动相,恒梯度洗脱,流速4.0 mL/min,柱温室温,检测波长为203 nm,在此色谱条件下,它们得率分别为0.012%、0.013%、0.012%、0.013%。该方法操作简便,可重复进样,适用于制备高纯度稀有人参皂苷20(S)-Rg3、20(R)-Rg3、Rg5、Rk1。
To establish a simple, rapid and efficient method for the preparation of ginsenoside 20 (S) -Rg3,20 (R) -Rg3, Rg5, Rk1. The sample was pretreated by macroporous adsorption resin and silica gel column chromatography, and the target product ginsenoside 20 (S) -Rg3,20 (R ) -Rg3, Rg5, Rk1, tested, the purity of four compounds were> 98%. The column was Polaris C18 (250 mm × 4.6 mm, 10 μm). The mobile phase consisted of V (acetonitrile): V (water) = 46:54 with a constant gradient of 4.0 mL / 203 nm. Under these chromatographic conditions, their yields were 0.012%, 0.013%, 0.012% and 0.013%, respectively. The method is simple and convenient to operate and can be repeatedly injected. It is suitable for preparing high purity rare ginsenoside 20 (S) -Rg3,20 (R) -Rg3, Rg5, Rk1.