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目的研究西妥昔单抗(C225)对不同照射模式人鼻咽低分化鳞癌细胞(CNE-2)裸鼠移植瘤细胞凋亡相关基因Bax、Bcl-2表达的影响。方法成功构建48只CNE-2裸鼠移植瘤模型,待成瘤后随机分为空白对照组、急速照射组、模拟IMRT组、C225组、急速照射+C225组、模拟IMRT+C225组,运用RT-PCR法检测Bax、Bcl-2的转录水平。结果 Bax转录水平:空白对照组、急速照射组、模拟IMRT组、C225组、急速+C225组和模拟IM-RT+C225组转录水平分别为0.452、0.662、0.558、0.542、0.621、0.555,放疗后Bax表达上调,急速照射组表达高于模拟IMRT组(P=0.005);使用C225后Bax表达上调(P=0.015);急速与急速+C225组、模拟IMRT组与模拟IMRT+C225组差异均无统计学意义(P>0.05)。Bcl-2转录水平:空白对照组、急速照射组、模拟IMRT组、C225组、急速+C225组和模拟IMRT+C225组转录水平分别为0.629、0.685、0.654、0.625、0.658、0.648,各组差异均无统计学意义(P>0.05)。结论模拟IMRT照射时间延长,影响Bax的表达;C225可能具有一定促凋亡作用;放疗联合C225同单独使用放疗相比Bax表达无明显差异;照射及应用C225后Bcl-2表达的相对稳定,不受照射方式及C225的影响。
Objective To investigate the effect of cetuximab (C225) on the expression of apoptosis-related genes Bax and Bcl-2 in xenografted tumor cells of human nasopharyngeal poorly differentiated squamous cell carcinoma (CNE-2) in different irradiation modes. Methods Forty-eight nude mice models of CNE-2 xenografts were successfully established and randomly divided into blank control group, rapid irradiation group, simulated IMRT group, C225 group, rapid irradiation + C225 group, simulated IMRT + C225 group, -PCR assay Bax, Bcl-2 transcription level. Results The transcription level of Bax in control group, rapid irradiation group, simulated IMRT group, C225 group, rapid + C225 group and simulated IM-RT + C225 group were 0.452,0.662,0.558,0.542,0.621,0.555 respectively. After radiotherapy The expression of Bax was up-regulated in acute radiation group and higher than that in simulated IMRT group (P = 0.005). The expression of Bax was up-regulated in C225 group (P = 0.015). There was no significant difference between rapid and rapid C2 C2 group, simulated IMRT group and simulated IMRT + C225 group Statistical significance (P> 0.05). The transcription level of Bcl-2 in the blank control group, rapid irradiation group, simulated IMRT group, C225 group, rapid + C225 group and simulated IMRT + C225 group were 0.629,0.685,0.654,0.625,0.658,0.648, respectively No statistical significance (P> 0.05). CONCLUSION: Imitation of IMRT prolongs the exposure time and affects the expression of Bax. C225 may have a pro-apoptotic effect. There is no significant difference in the expression of Bax between radiotherapy combined with C225 and radiotherapy alone. The expression of Bcl-2 is relatively stable after irradiation and after C225 irradiation Affected by the way of exposure and C225.