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用免疫吸附剂纯化抗原、抗体或其它生物大分子是亲和层析法中的重要内容之一。它的依据是抗原(或抗体)可以和它们相应的配体(ligand)——抗体(或抗原)进行专一的结合,而这种结合在一定条件下是可逆的。若将抗原(或抗体)固相化后,就可使与之接触的溶液中的抗体(或抗原)被结合在固相配体上,从而达到分离提纯的目的。溴化氰活化琼脂糖微球是一种常用的方法,其步骤如下:
Purification of antigens, antibodies or other biological macromolecules with immunoadsorbents is an important aspect of affinity chromatography. It is based on the specific binding of antigens (or antibodies) to their corresponding ligands - antibodies (or antigens), which under certain conditions are reversible. If the antigen (or antibody) is immobilized, the antibody (or antigen) in the solution contacted with the antibody (or antigen) can be bound to the solid phase ligand to achieve the purpose of isolation and purification. Cyanide bromide activated agarose microspheres is a commonly used method, the steps are as follows: