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目的:探讨核转录因子(NF-κB)、胎盘生长因子(PLGF)、肿瘤坏死因子(TNF-α)与子痫前期发病的关系,为临床诊断及治疗提供依据。方法:选取60例子痫前期患者(观察组,其中轻度30例,重度30例)和30例正常孕妇(对照组),采用酶联免疫吸附法(ELISA)对观察组和对照组患者的血清TNF-α和PLGF水平以及胎盘中NF-κB、TNF-α和PLGF水平进行检测,对三者的相关性进行分析。结果:观察组患者胎盘组织中TNF-α和NF-κB表达水平显著高于对照组(P<0.05),观察组患者血清PLGF表达水平显著低于对照组(P<0.05)。观察组中重度子痫前期组患者TNF-α和NF-κB表达水平显著低于轻度子痫前期组(P<0.05),二者PLGF表达水平相比无统计学差异(P<0.05)。且NF-κB及TNF-α与PLGF表达强度呈显著负相关,NF-κB与TNF-α表达强度呈显著正相关。观察组患者血清TNF-α水平显著高于对照组(P<0.05),观察组患者血清PLGF的水平显著低于对照组(P<0.05)。观察组中重度子痫前期组患者血清TNF-α水平较轻度子痫前期组显著增高(P<0.05),血清PLGF水平显著低于轻度子痫前期组(P<0.05),且观察组患者血清TNF-α与PLGF水平呈显著负相关。结论:在子痫前期发生过程中,TNF-α、PLGF、NF-κB三种细胞因子间存在一定的联系,可能通过相关机制参与子痫前期发病,并且三类物质与子痫前期的进展及其病情严重程度有密切关系。
Objective: To investigate the relationship between the expression of NF-κB, placental growth factor (PLGF) and tumor necrosis factor (TNF-α) and the pathogenesis of preeclampsia and provide the basis for clinical diagnosis and treatment. Methods: 60 cases of preeclampsia (observation group, 30 cases of mild, 30 cases of severe) and 30 cases of normal pregnant women (control group) were selected. The serum of patients in observation group and control group was detected by enzyme-linked immunosorbent assay (ELISA) TNF-α and PLGF levels and placental NF-κB, TNF-α and PLGF levels were detected, the correlation between the three were analyzed. Results: The expression of TNF-α and NF-κB in the placenta of the observation group was significantly higher than that of the control group (P <0.05). The expression of PLGF in the observation group was significantly lower than that of the control group (P <0.05). The levels of TNF-α and NF-κB in the patients with severe preeclampsia in the observation group were significantly lower than those in the mild preeclampsia group (P <0.05). There was no significant difference in the expression of PLGF between the two groups (P <0.05). The expressions of NF-κB, TNF-α and PLGF were negatively correlated with each other. The expression of NF-κB was positively correlated with the expression of TNF-α. The level of serum TNF-α in the observation group was significantly higher than that in the control group (P <0.05). The level of serum PLGF in the observation group was significantly lower than that in the control group (P <0.05). The levels of TNF-α in serum of patients with severe preeclampsia in the observation group were significantly higher than those in the mild preeclampsia group (P <0.05), and the levels of serum PLGF in the severe preeclampsia group were significantly lower than those in the mild preeclampsia group (P <0.05) Serum TNF-α and PLGF levels were significantly negatively correlated. CONCLUSIONS: During the development of preeclampsia, TNF-α, PLGF and NF-κB may be involved in the pathogenesis of preeclampsia through the related mechanisms, and the progress of these three substances and preeclampsia The severity of the disease is closely related.