反相 HPL C法测定人血浆及尿中溴莫普林浓度。血浆及尿样经二氯甲烷萃取等预处理后 ,于Symmetry C1 8柱上 ,以甲醇 - 0 .0 2 mol/ L NH4H2 PO4(6 0 :40 ,p H7.6 0 )为流动相进行分离测定。线性范围 0 .0 5～4mg/ L,萃取回收率 82 .4%～ 86 .1% ,加样回收率 96 .6 %～ 10 0 .9% ,日内 RSD1.72 %～ 3.81% ,日间 RSD3.10 %～ 7.6 3%。本法准确可靠 ,适于在溴莫普林药动学等研究中应用。 Determination of Bromotripton in Human Plasma and Urine by Inverse HPL C Method. Plasma and urine samples were pretreated with methylene chloride and then separated on a Symmetry C1 8 column using methanol-0.02 mol / L NH4H2PO4 (60:40, p H7.6 0) as the mobile phase Determination. The linear range was from 0.05 to 4 mg / L, the extraction recovery was 82.4% ~ 86.1%, the recovery was 96.6% -10.9%, and the intraday RSD was 1.72% -3.81% RSD3.10% ~ 7.6 3%. This method is accurate and reliable, suitable for research in the pharmacokinetics of bromophorin and other applications.