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作者评价了用纯化的呼吸道合胞病毒（RSV）融合(F）蛋白和霍乱毒素（CT）佐剂疫苗粘膜免疫接种小鼠的保护效力。 取4周龄小鼠进行实验。用于鼻内接种的10μl疫苗中含CT2.5μg、F蛋白3μg;肌肉接种的0.1ml明矾疫苗中含F蛋白3μg,用PBS作安慰剂。活病毒疫苗为2.5μl中含2×10~6空斑形成单位（PFU）病毒。在0和4周各免疫1次,8周时用RSV Long株5×10~6PFU攻击。病毒攻击后4天杀死小鼠,取血清,用酶免疫试验测定抗F蛋白和CT的IgG抗体,并作微量中和试验。无菌取鼻洗液,一部分立刻用HEp-2细胞滴定病毒滴度,一部分测定IgA。 动物共分9组,每组6只。实验结果表明,鼻内接种活RSV组血清中有高滴度抗F特异IgG具有强的中和力,鼻洗液中有IgA,病毒攻击后,所有小鼠均未检出病毒。鼻内和肌肉同时接种PBS组,无抗体产生,不能阻止病毒复制,鼻内病毒滴度为log_210.1±0.2PFU。鼻内或肌肉接种F蛋白组,鼻洗液中均无IgA,不能阻止病毒复制,但后者有血清抗体。鼻内和肌肉同时接种F蛋白组亦有高滴度血清抗体但无鼻洗液IgA,病毒复制力比PBS组低。鼻内单独接种CT组,虽无抗F抗体,但能轻度抑制病毒复制,鼻洗液中病毒滴度为log_26.8±2.2 PFU,每只小鼠皆查到病毒说明与免疫接种无关。鼻内接种F+CT组,血清中有高滴度F和CT特异性IgG并
The authors evaluated the protective efficacy of mucosal immunization of mice with purified respiratory syncytial virus (RSV) fusion (F) protein and cholera toxin (CT) adjuvant vaccine. Four-week-old mice were used for experiments. The 10 μl vaccine used for intranasal injections contained CT 2.5 μg and F protein 3 μg; the muscle inoculated 0.1 ml alum vaccine contained 3 μg F protein and PBS was given as a placebo. The live virus vaccine contains 2x10-6 plaque forming units (PFU) virus in 2.5μl. Each immunization was given at 0 and 4 weeks and challenged with 5 × 10 -6 PFU of RSV Long strain at 8 weeks. The mice were sacrificed 4 days after the virus challenge, serums were taken and IgG antibodies against F protein and CT were determined by enzyme immunoassay and a minimal neutralization test was performed. Aseptic nasal wash, part of the titer immediately titrated with HEp-2 cells, part of the determination of IgA. The animals were divided into 9 groups, 6 in each group. The experimental results showed that high titer anti-F specific IgG in sera of intranasal RSV vaccination had strong neutralizing force and IgA in nasal wash. No virus was detected in all mice after virus challenge. Intranasal and muscle inoculation PBS group, no antibody, can not stop the virus replication, intranasal virus titer log_210.1 ± 0.2PFU. Intranasal or muscle inoculation F protein group, nasal wash no IgA, can not stop the virus replication, but the latter has serum antibodies. Inoculation of intranasal and intramuscular F protein group also had high titers of serum antibody but no nasal wash IgA, viral replication was lower than PBS group. Intranasal inoculation alone CT group, although no anti-F antibody, but could slightly inhibit the virus replication, nasal wash virus titer of log_26.8 ± 2.2 PFU, each mouse found that the virus has nothing to do with the immunization. Intranasal vaccination F + CT group, serum high titer F and CT-specific IgG and