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目的观察高氟区和非高氟区人牙胚成釉细胞形态变化及凋亡相关基因bc l-2及bax的表达。方法经知情同意在高氟区及非高氟区分别取引产胎儿牙胚设为试验组与对照组,组织学观察,免疫组化技术检测成釉细胞中bc l-2及bax的表达,测定母体尿氟。结果试验组母体尿氟含量高于正常范围(P<0.01),成釉细胞排列不规则,高度变矮,少量细胞发生扭曲,并在细胞质内有空泡产生,TUNEL检测显示有较多凋亡细胞,与对照组相比bc l-2表达减弱,bax表达增强。结论高氟导致人牙胚成釉细胞损伤及凋亡。高氟对成釉细胞的凋亡作用可能是通过调节Bc l-2和Bax表达机制诱导细胞凋亡发生。
Objective To observe the morphological changes of ameloblasts and the expressions of bcl-2 and bax in human dental germ with and without high fluoride. Methods Totally fetus embryos were fetched into experimental and control groups respectively by informed consent in high fluoride area and non-high fluoride area. The expression of bcl-2 and bax in ameloblasts was detected by immunohistochemistry, Urine fluoride Results The urinary fluoride content of the experimental group was higher than the normal range (P <0.01). The ameloblasts were arranged irregularly, the height was short, a small amount of cells were distorted, vacuoles were generated in the cytoplasm, and TUNEL assay showed more apoptosis Cells, compared with the control group bc l-2 expression decreased, bax expression increased. Conclusion High fluoride causes apoptosis and apoptosis of ameloblasts in human tooth germ. Apoptosis induced by high fluoride on ameloblasts may be induced apoptosis by regulating Bcl-2 and Bax expression.