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梗阻性黄疸易并发消化性溃疡,其中机制尚未明了,但认为与多种因素有关,我们通过测定梗阻性黄疸的大鼠血清胃泌素水平,从一个侧面阐述其中的机制。 材料和方法 一、动物与分组:选用本院动物房提供的60只年龄相近、体重200～300g、饲养条件一致的SD大鼠,雌雄不拘,随机分为二组:手术组,假手术组。每组各30只。所有大鼠在清晨空腹时从尾部取血1～2ml,待试管中血凝固后,离心分离血清,置-4℃保存待测。 二、梗阻性黄疸大鼠模型的制作:将手术组动物30只在1％硫喷妥钠腹腔麻醉下(剂量按3.5mg/100g)消毒腹部,取下中切口,依层切开腹腔,在肝十二指肠韧带内找到胆总管,双重结扎,关腹。假手术组只分离胆总管而不结扎。所有动物
Obstructive jaundice is complicated by peptic ulcer, the mechanism of which is unclear, but considered to be related to a variety of factors, we measure the serum gastrin levels of obstructive jaundice in rats and elucidate the mechanism from one aspect. MATERIALS AND METHODS A. Animals and Subgroups: Sixty SD rats of similar ages and weighing 200-300 g and rearing conditions provided by our hospital were randomly divided into two groups: operation group and sham operation group. Each group of 30. All rats in the early morning fasting blood from the tail 1 ~ 2ml, until the blood coagulation in the test tube, centrifuged serum, set at -4 ℃ to be tested. Second, obstructive jaundice rat model: 30 animals in the operation group were treated with 1% thiopental sodium intraperitoneal anesthesia (dose according to 3.5mg / 100g) disinfection of the abdomen, remove the incision, according to cut the abdominal cavity, in the Liver duodenum ligament to find the common bile duct, double ligation, close the abdomen. Sham-operated group only separated the common bile duct without ligation. All animals