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为了探索SUMO E3连接酶(SIZ1)基因与桃(Prunus persica L.Batsch)果实采后低温贮藏期间冷害发生的关系,对桃基因组中的SIZ1基因进行生物信息学分析,同时采用qRT-PCR分析该基因在‘湖景蜜露’桃果实低温贮藏以及外源褪黑素减轻冷害进程中的表达特征。结果表明,Pp SIZ1开放阅读框全长2 637 bp,编码878个氨基酸组成的蛋白质多肽。进化树分析发现,PpSIZ1与梅Pm SIZ1的同源性最高,含有与拟南芥At SIZ1相似的SAP、PHD、PINIT、SP-RING和SXS保守结构域,属于SUMO E3连接酶家族。q RT-PCR分析表明,低温(0℃)胁迫能诱导桃果实PpSIZ1表达水平的提高;100和200μmol·L~(-1)褪黑素处理能显著减轻桃果实低温贮藏期间冷害的发生,其中100μmol·L~(-1)褪黑素处理抑制冷害的效果最好,这种效果可能与PpSIZ1介导的SUMO化有关。
In order to explore the relationship between SUMO E3 ligase (SIZ1) gene and postharvest chilling injury in postharvest fruit of Prunus persica L. Batsch, bioinformatics analysis was performed on the SIZ1 gene in peach genome and analyzed by qRT-PCR Gene Expression Characteristics in ’Lake View Honeydew’ Peach Fruit Cold Storage and Exogenous Melatonin Alleviating Chilling Injury. The results showed that the Pp SIZ1 open reading frame was 2 637 bp in length and encoded a protein polypeptide consisting of 878 amino acids. Phylogenetic analysis showed that PpSIZ1 shared the highest homology with Pm SIZ1 and contained the conserved domains of SAP, PHD, PINIT, SP-RING and SXS similar to At SIZ1 in Arabidopsis, belonging to the SUMO E3 ligase family. q RT-PCR analysis showed that cold stress (0 ℃) induced the increase of PpSIZ1 expression in peach fruit; 100 and 200μmol·L -1 melatonin treatment could significantly reduce the cold damage during peach fruit storage at low temperature, of which The effect of 100μmol·L -1 melatonin on chilling injury was the best, which may be related to PpSIZ1-mediated SUMOylation.