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以黄瓜根际土壤为试材,采用亚历山大硅酸盐细菌培养基从黄瓜根际土壤中分离筛选解钾菌,用原子吸收法测定菌株的解钾能力,选出解钾能力较强的菌株进行菌种鉴定,同时研究其对黄瓜幼苗生长的影响。结果表明:从黄瓜根际土壤中共分离获得9株细菌,其中PK1、PK3和PK7具有较强的解钾能力,液体培养96h后其解钾率分别为30.05%、33.78%和44.01%。依据菌株的形态学特征和生理生化特征鉴定,PK1和PK3为恶臭假单胞菌(Pseudomones putida),PK7为胶质类芽孢杆菌(Paenibacillus mucilaginosus)。盆栽试验结果表明,与对照相比,14d后PK1提高黄瓜的根长、株高、鲜质量和干质量分别为42.60%、10.45%、48.65%、33.01%,PK3分别对应提高65.26%、21.09%、83.78%和49.48%,PK7分别对应提高79.46%、44.26%、105.41%和113.03%。
The cucumber rhizosphere soil was used as test material, and the potassium silicate bacteria were isolated and screened from rhizosphere soil of cucumber by using silicate silicate culture medium. The potassium-releasing ability of the strain was determined by atomic absorption spectrometry. Strain identification, at the same time to study the cucumber seedling growth. Nine bacterial strains were isolated from rhizosphere soil of cucumber. Among them, PK1, PK3 and PK7 had strong ability to dissolve potassium, and the rates of potassium dissolution after liquid culture for 96h were 30.05%, 33.78% and 44.01%, respectively. According to the morphological and physiological and biochemical characteristics of the strains, PK1 and PK3 were Pseudomonas putida and PK7 was Paenibacillus mucilaginosus. The results of pot experiment showed that the root length, plant height, fresh weight and dry weight of cucumber increased by 42.60%, 10.45%, 48.65% and 33.01%, respectively, and that of PK3 increased by 65.26% and 21.09% , 83.78% and 49.48%, respectively. PK7 increased by 79.46%, 44.26%, 105.41% and 113.03% respectively.