常用的微核试验方法系用受试物对动物于0和24小时给药二次,并于第一次给药后30小时取材。但取材时间和给药次数很可能影响微核的发生率。作者对五种诱变剂的微核时间反应进行观察,企图寻找适当的取材时间,并对九种诱变剂的一次给药方法与五次给药方法的微核发生率进行了比较,以了解重复处理对微核发生率的影响。片子分析:每一只鼠分析1000个红细胞(包括嗜多染和成熟红细胞)的微核数,并将带有微核的红细胞分为4型,根据胞浆的颜色和微核的相对大小分别记录。作者认为此种分析 Commonly used method of micronucleus test is that the animals are administered twice with the test substance at 0 and 24 hours, and drawn 30 hours after the first administration. However, the time and number of administration is likely to affect the incidence of micronuclei. The authors observed the micronucleus time response of five mutagens in an attempt to find out the appropriate time of drawing, and compared the incidence of micronuclei in five administrations of nine mutagens and Understand the impact of repetitive treatments on the incidence of micronuclei. Film analysis: Each mouse analysis of 1000 red blood cells (including polycytosis and mature erythrocytes) micronuclei and micronucleated erythrocytes are divided into 4 types, according to the size of the cytoplasm and the relative size of the micronucleus recording. The author thinks this kind of analysis