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目的研究镇痛灵对Wistar糖尿病大鼠的防治作用机制。方法3～5个月龄Wistar鼠75只,雌雄不限,随机分为糖尿病组60只,尾静脉推注1.5%四氧嘧啶50mg/kg;正常对照组15只,尾静脉注射等体积生理盐水。72h后,以血糖值大于16.67mmol/L为造模成功。将糖尿病大鼠随机分为4组:①模型组;②3种不同剂量镇痛灵治疗组。第5周测血糖后乙醚麻醉下断头取血,分离血清测放免、生化指标。结果①1型糖尿病模型制备成功;②糖尿病大鼠与正常对照组比较,血清胰岛素(Ins)、C-肽、T-AOC明显降低,差异有高度显著性意义(P<0.001);血糖、XO值升高(P<0.001);③中、高剂量治疗组血清血清胰岛素(Ins)、C-肽、T-AOC值与模型组比较,明显升高,差异有统计学意义(P<0.05);血糖、XO值降低(P<0.05)。低剂量治疗组各指标变化与模型组比较,差异无统计学意义(P>0.05)。结论镇痛灵对糖尿病有防治作用,并在一定剂量范围内呈量效依赖关系。
Objective To study the preventive and therapeutic mechanism of “Analgesic” on Wistar diabetic rats. Methods Seventy-five Wistar rats of 3 to 5 months old were randomly divided into diabetic group (n = 60), caudal vein injection of 1.5% alloxan 50 mg / kg, normal control group (n = 15) and tail vein injection of equal volume of normal saline . After 72h, the blood glucose value is greater than 16.67mmol / L for modeling success. The diabetic rats were randomly divided into 4 groups: ① model group; ③ 3 different doses of analgesic treatment group. After 5 weeks of blood glucose test under ether anesthesia decapitated blood, serum test-free, biochemical indicators. Results ① The model of type 1 diabetes was successfully established. ② The levels of serum insulin, C-peptide and T-AOC in diabetic rats were significantly lower than those in normal controls (P <0.001) (P <0.001). ③The levels of serum insulin, C-peptide and T-AOC in middle-dose and high-dose treatment groups were significantly higher than those in model group (P <0.05); Blood glucose, XO value decreased (P <0.05). There was no significant difference between the low-dose treatment group and the model group (P> 0.05). Conclusion Analgesic has a preventive and therapeutic effect on diabetes and is dose-dependent in a certain dose range.