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[方法]为明确乙酰甲胺磷.噻虫嗪水乳剂、水分散粒剂和毒死蜱.噻虫嗪水分散粒剂3种新型复配剂的增效作用及增效机理,室内采用喷雾法测定了对菜青虫的增效作用;通过生化测定法研究了其对菜青虫体内羧酸酯酶(CarE)、谷胱甘肽S-转移酶(GSTs)和乙酰胆碱酯酶(AChE)活力的影响;采用石蜡切片显微观察法分析了3种复配剂处理后菜青虫中肠组织细胞的变化。[结果]3种复配剂对菜青虫都有很好的杀虫活性,处理48 h后的共毒系数分别为131.10、459.04、204.94,都表现为明显的增效作用;乙酰甲胺磷.噻虫嗪水乳剂显著抑制CarE、GSTs和AChE活力,抑制率分别为90.04%、74.98%、71.59%;乙酰甲胺磷.噻虫嗪水分散粒剂对3种酶活力的抑制率分别为72.11%、74.98%、90.60%;毒死蜱.噻虫嗪水分散粒剂则仅抑制CarE和GSTs活力,抑制率为71.16%和88.55%;3种复配剂明显影响菜青虫中肠肠壁细胞的层次结构以及肠壁细胞中的杯状细胞形状及结构。[结论]3种新型复配剂可能是通过抑制菜青虫体内关键酶活性和破坏中肠组织细胞形状和结构,进而影响其正常的消化和吸收功能,达到增效和杀虫的目的。
[Method] In order to clarify the synergism and synergistic mechanism of three novel compounding agents of acephate, thiamethoxam, water dispersible granules and chlorpyrifos, and thiamethoxam water dispersible granules, The synergistic effect on Pieris rapae was studied. The effect of CarE, Carboxylesterase (GSTs) and acetylcholinesterase (AChE) on the activity of Pieris rapae was studied by biochemical assay. The changes of midgut tissue cells of Pieris rapae after three kinds of compounding agents were analyzed by paraffin section microscopic observation. [Result] The three kinds of compounding agents all had good insecticidal activity against Pieris rapae. The co-toxicity coefficients after 48 hours of treatment were 131.10, 459.04 and 204.94, respectively, showing obvious synergistic effects. Thiamethoxam water emulsion significantly inhibited CarE, GSTs and AChE activity, the inhibition rates were 90.04%, 74.98%, 71.59%; Acephate. Thiamethoxam water dispersible granules on the three enzyme activity inhibition rates were 72.11 %, 74.98% and 90.60%, respectively. Chlorpyrifos and thiamethoxam respectively inhibited the activity of CarE and GSTs with the inhibitory rates of 71.16% and 88.55%, respectively. Structure and goblet cells in the goblet cells in the shape and structure. [Conclusion] The three new types of compounding agents may affect synergistic and insecticidal effects by inhibiting the key enzyme activity in cabbage beetles and destroying the shape and structure of midgut tissue cells, thereby affecting their normal digestion and absorption functions.