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目的探讨不同种类氨基酸对神经胶质抗原2(NG2)蛋白聚糖阳性神经祖细胞(NG2细胞)增殖的作用。方法从成年大鼠脑不同区域分离培养NG2细胞,以0.1-0.5mmol/L浓度不同种类氨基酸干预细胞72小时,以乳酸脱氢酶(LDH)分析法测定细胞活性。结果 0.3mmol/L谷氨酸和天冬氨酸显著增加不同脑区来源NG2细胞数(p<0.001)。结论高浓度兴奋性氨基酸(EAAs)能促进NG2细胞的增殖。
Objective To investigate the effect of different kinds of amino acids on the proliferation of NG2 proteoglycan positive neural progenitor cells (NG2 cells). Methods NG2 cells were isolated and cultured from different regions of adult rat brain. The cells were treated with different concentrations of amino acids (0.1-0.5 mmol / L) for 72 hours. The cell viability was measured by lactate dehydrogenase (LDH) assay. Results 0.3mmol / L glutamate and aspartate significantly increased the number of NG2 cells in different brain regions (p <0.001). Conclusion High concentrations of excitatory amino acids (EAAs) can promote the proliferation of NG2 cells.