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目的:研究不同类型的尿道下裂尿道板和包皮中的来源于HOX家族的HoxA13基因转录及蛋白表达,以探讨HoxA13在尿道下裂发病的作用。方法:取30例(3.3~11.6岁)尿道下裂患儿包皮及尿道板以及10例(3.1~10.4岁)包茎患儿包皮和10例(48.1~75.6岁)阴茎癌尿道未受侵犯患者的尿道作为对照。分为尿道下裂前型、中间型、后型及对照组,每组10例。采用逆转录PCR和免疫组化检测包皮与尿道板组织中的HoxA13的转录水平和蛋白表达量。结果:逆转录PCR:HoxA13 mRNA表达水平在对照组(包皮:1.409±0.441,尿道板:1.270±0.209)明显高于中间型(包皮:0.848±0.338,尿道板:0.684±0.228)和后型(包皮:0.497±0.218,尿道板:0.464±0.164)(P<0.05或P<0.01);前型(包皮:1.071±0.342,尿道板:1.054±0.189)表达水平明显多于后型(P<0.05)。免疫组化检测:HoxA13蛋白表达水平在对照组(包皮:12 050±4 112,尿道板:13 420±2 636)明显高于中间型(包皮:5 217±1 993,尿道板:5 238±3 065)和后型(包皮:2 095±1 591,尿道板:2 238±2 217)(P<0.01);前型(包皮:8 223±3 212,尿道板:10 450±2 123)表达水平明显高于后型(P<0.05)。结论:尿道下裂患者的包皮和尿道板中HoxA13的转录及表达量与尿道异常开口位置有关,这种异常的表达可能影响尿道的发育和成型。
OBJECTIVE: To study the transcription and protein expression of HoxA13 gene in HOX family in different types of hypospadias urethral plate and prepuce, in order to investigate the role of HoxA13 in the pathogenesis of hypospadias. Methods: Thirty cases (3.3 to 11.6 years old) of children with hypospadias foreskin and urethral plate and 10 cases (3.1 to 10.4 years) phimosis circumcision and 10 cases (48.1 ~ 75.6 years) of penile cancer patients without invasion of the urethra Urinary tract as a control. Divided into pre-hypospadias, intermediate, post-type and control group, 10 cases in each group. Reverse transcriptase PCR and immunohistochemistry were used to detect the transcriptional level and protein expression of HoxA13 in foreskin and urethral plate tissues. Results: The mRNA expression of HoxA13 in reverse transcription PCR was significantly higher in the control group (foreskin: 1.409 ± 0.441, urethral plate: 1.270 ± 0.209) than in the intermediate type (foreskin: 0.848 ± 0.338, urethral plate: 0.684 ± 0.228) Foreskin: 0.497 ± 0.218, urethral plate: 0.464 ± 0.164) (P <0.05 or P <0.01). The former type (foreskin: 1.071 ± 0.342, urethral plate: 1.054 ± 0.189) ). The expression of HoxA13 protein in the control group (foreskin: 12 050 ± 4 112, urethral plate: 13 420 ± 2 636) was significantly higher than that in the intermediate type (foreskin 5 217 ± 1 993, urethral plate 5 238 ± (Foreskin: 823 ± 3 212, urethra plate: 10 450 ± 2 123) and posterior (foreskin: 2 095 ± 1 591, urethral plate: 2 238 ± 2 217) The expression level was significantly higher than the posterior type (P <0.05). CONCLUSIONS: The transcription and expression of HoxA13 in the foreskin and urethral plate of patients with hypospadias are related to the location of the abnormal urethral opening. This abnormal expression may affect the development and formation of the urethra.