论文部分内容阅读
为探讨茶叶糖蛋白(TGP)对小鼠骨髓来源树突状细胞(DCs)表型及功能的影响,采用细胞因子诱导法,以贴壁法获得贴壁单核细胞,添加重组粒细胞-巨噬细胞集落刺激因子(rmGM-CSF)和重组白细胞介素-4(rmIL-4)进行体外诱导培养,倒置显微镜动态观察细胞形态的变化;采用流式细胞术检测DCs的表面标志CD11c和MHCII类分子表达;采用MTT法检测TGP对DCs刺激OVA未致敏或致敏淋巴细胞增殖的影响;初步探讨TGP对DCs抗小鼠SP2/0骨髓瘤功能的影响。结果表明,经TGP作用48h后,树突状细胞形态更加典型、成熟;与阴性对照相比,TGP显著促进树突状细胞表面CD11c和MHCⅡ的表达;TGP可增强DCs的刺激致敏淋巴细胞增殖的能力,说明DCs诱导免疫应答及抗原提呈功能均增强;与未经抗原负载相比,TGP抗肿瘤机制与促进DCs抗原呈递能力有密切的关系,可提高机体对肿瘤细胞的特异性主动免疫功能,而TGP的干预可促进这一功能的提高。茶叶糖蛋白可以促进树突状细胞表型及功能的成熟。
To explore the effect of tea glycoprotein (TGP) on the phenotype and function of mouse bone marrow-derived dendritic cells (DCs), the adherent mononuclear cells were obtained by adherent method using cytokine induction method, The cultured cells were induced by rmGM-CSF and rmIL-4. The morphological changes of cells were observed by inverted microscope. The surface markers CD11c and MHC class II of DCs were detected by flow cytometry The effect of TGP on DCs anti-mouse SP2 / 0 myeloma function was investigated by MTT assay. The results showed that the morphology of dendritic cells was more typical and matured after TGP treatment for 48h. Compared with the negative control, TGP significantly promoted the expression of CD11c and MHCⅡ on the surface of dendritic cells; TGP enhanced the proliferation of dendritic cells stimulated by DCs , Indicating DCs induced immune response and antigen presentation were enhanced; compared with non-antigen load, TGP anti-tumor mechanism and the promotion of antigen presenting ability of DCs are closely related to improve the body’s specific active immune cells Function, and TGP intervention can promote this function to improve. Tea glycoprotein can promote the maturation of dendritic cell phenotype and function.