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作者从100公斤湿重的印度洋海兔中分得约各为lmg的9种抗癌或细胞毒素成份,并阐明了其中一种成份,海兔抑制素3(Dolastatin 3)的化学结构。海兔抑制素3为无色、无定形固体,熔点133～187℃,[α]_D~(26)—35.5℃(C=0.09, 甲醇)。根据光谱行为,IR,高分辨NMR~1H和~(13)C谱推断为多肽类物质,氨基酸分析测定含有1:1:1克分子比的脯氨酸、缬氨酸、亮氨酸和二种未知氨基酸,经6.1NHCl水解继以甲基化、乙酰化和气相质谱分析除得到脯氨酸、缬氨酸和亮氨酸的N-乙酰基甲酯外,还有两种未知氨基酸的N-乙酰基甲酯,证明只有5种氨基酸组分。根据元素
The authors obtained approximately 1 mg of anti-cancer or cytotoxin ingredients at about 1 mg each from a 100 kg wet Indian Indian sea-hare and elucidated the chemical structure of one of the components, Dolastatin 3. Rabbit inhibin 3 is a colorless, amorphous solid, m.p. 133-187 ° C, [α] D 26 (-35.5 ° C (c = 0.09, methanol). Based on the spectroscopic behavior, IR, high resolution NMR ~ 1H and ~ (13) C spectra, it was deduced to be a polypeptide substance. Amino acid analysis determined proline, valine, leucine and di In addition to N-acetylmethyl esters of proline, valine and leucine, hydrolysis of 6.1N HCl followed by methylation, acetylation and gas-phase mass spectrometry analysis revealed two unknown amino acids, N - Acetyl methyl ester, proving that there are only 5 kinds of amino acid components. According to the element