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本研究以梭柄松苞菇为载体,亚硒酸钠为硒源,分析评价了在两种富硒培养方法(固体培养和液体培养)下,硒浓度对梭柄松苞菇的生产效率、富硒量、有机硒转化率以及单位干重中有机硒含量的影响。结果表明,在最适硒浓度条件下,固体培养时,每千克培养基可收获91.30 g子实体,富硒量达到69.8μg/g,有机硒转化率为92.60%,有机硒含量为64.63μg/g;而液体培养时,每升培养液可收获4.50 g的菌丝,富硒量达到199.50μg/g,有机硒转化率为84.60%,有机硒含量为168.78μg/g。同时分别测定了梭柄松苞菇的富硒子实体多糖(SFCs)、富硒菌丝多糖(SPCs)、未经富硒的子实体多糖(FCs)和菌丝多糖(PCs)的体外降血糖活性和抗氧化活性,结果表明SFCs和SPCs对硒元素的富集,可能是其降血糖和抗氧化活性高于FCs和PCs的重要原因,并且SPCs的降血糖和抗氧化活性均显著高于阳性对照。
In this study, the seedlings of B. japonicus were treated with sodium selenite as the selenium source. The production efficiency of selenium in this plant was analyzed and evaluated under two selenium-enriched cultivation methods (solid culture and liquid culture) Selenium content, conversion of organic selenium and organic selenium content per unit dry weight. The results showed that 91.30 g fruiting bodies could be harvested per kilogram of culture medium under the optimized conditions of selenium concentration, the selenium content reached 69.8 μg / g, the organic selenium conversion rate was 92.60%, the organic selenium content was 64.63 μg / g, and 4.50 g of mycelium per liter of culture liquid could be harvested when the liquid was cultured. The amount of selenium was 199.50μg / g, the organic selenium conversion was 84.60% and the organic selenium was 168.78μg / g. At the same time, the hypoglycemic activity of polysaccharide (SFCs), Se-enriched mycelium polysaccharides (SPCs), fructus fructus polysaccharides (FCs) and mycelial polysaccharides (PCs) Activity and antioxidant activity of SFCs and SPCs. The results showed that the enrichment of selenium by SFCs and SPCs may be an important reason for its hypoglycemic and antioxidant activities higher than FCs and PCs, and the hypoglycemic and anti-oxidative activities of SPCs were significantly higher than those of positive Control.