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目的:研究缺氧诱导因子-1α(HIF-1α)、碳酸酐酶Ⅸ(CAⅨ)在鼻息肉组织中的表达及其相关性,探讨HIF-1α、CAⅨ在鼻息肉发病过程中的作用。方法:收集28例鼻息肉组织(鼻息肉组)和12例正常下鼻甲组织(对照组),用免疫组织化学法检测鼻息肉组与对照组中HIF-1α与CAⅨ的表达,观察比较2组之间表达的差异。结果:免疫组织化学染色显示鼻息肉组HIF-1α、CAⅨ免疫阳性细胞数及着色强度均明显高于对照组。图像分析显示,鼻息肉组HIF-1α、CAⅨ的积分吸收度值(103/HP)分别为21.76±3.52、26.87±4.60,与对照组3.37±1.65、3.25±1.20相比差异有统计学意义(P<0.05);Spearman等级相关分析显示,鼻息肉组中HIF-1α表达与CAⅨ表达密切相关(r=0.820,P<0.01)。结论:鼻息肉组中HIF-1α、CAⅨ表达均上调,且二者有协同表达关系,说明鼻息肉组织中存在缺氧现象,再加上感染、炎症等作用使氧耗增多,能量代谢失调,加重局部缺氧,HIF-1α表达升高,并且能够激活、上调CAⅨ的表达,从而在缺氧状况下维持细胞的正常pH值,使细胞适应缺氧微环境,从而保证缺氧区域细胞的持续生长和增殖,最终形成恶性循环,导致鼻息肉的形成。
OBJECTIVE: To study the expression of HIF-1α and carbonic anhydrase Ⅸ (CAIX) in nasal polyps and to investigate the role of HIF-1α and CAIX in the pathogenesis of nasal polyps. Methods: 28 cases of nasal polyps (nasal polyps) and 12 cases of normal inferior turbinate tissues (control group) were collected. The expression of HIF-1α and CAⅨ in nasal polyps and control group were detected by immunohistochemistry. The difference between the expression. Results: Immunohistochemical staining showed that the number of immunopositive cells and the staining intensity of HIF-1α and CAⅨ in nasal polyps were significantly higher than those in the control group. The results of image analysis showed that the integral absorption values of HIF-1α and CAIX in nasal polyps were (21.76 ± 3.52,26.87 ± 4.60, respectively), which were significantly different from those in control group (3.37 ± 1.65 and 3.25 ± 1.20) P <0.05). Spearman rank correlation analysis showed that HIF-1α expression in nasal polyps was closely related to CAⅨ expression (r = 0.820, P <0.01). CONCLUSIONS: The expression of HIF-1α and CAⅨ in nasal polyps group are up-regulated, and the expression of HIF-1α and CAⅨ is up-regulated in nasal polyps. The synergistic expression of the two proteins suggests that there is anoxia in nasal polyps. Combined with infection and inflammation, oxygen consumption increases, Increased hypoxia, increased expression of HIF-1α, and can activate and up-regulate the expression of CAⅨ, so as to maintain the normal pH value of cells under hypoxia condition and adapt the cells to the hypoxic microenvironment so as to ensure the sustained cells in the hypoxic area Growth and proliferation, eventually forming a vicious circle, resulting in the formation of nasal polyps.