Objective: To study the expressive characteristics of SKP2 protein in non-small cell lung carcinoma and it is affection to NSCLC patients’ prognosis. Methods: The expression of SKP2 protein was detected in 89 NSCLC, 5 benign lung neoplasmas, 5 normal bronchus and lung tissues by Tissue Chip and immunohistochemistry technology.Results: The positive rate of SKP2 protein staining was (23.52±13.57)% in NSCLC tissues, significantly higher than that in benign lung neoplasmas, normal brochus and lung tissues (2.91±1.27)% (P=0.0000＜0.001). The expressive level of SKP2 protein in NSCLC tissues was closely related to cell differentiation (P1=0.000＜0.001),but not to age, sex, smoking history, pathological type, site, size, lymph node metastasis and TNM stage (each P1＞0.05). The survival analysis displayed that the NSCLC patients’ 5 years survival rate was lower in positive expression group than that in negative expression group (P1=0.042/0.031＜0.05; r=-0.186, P2=0.000＜0.001).Conclusion: The positive expression of SKP2 protein may play an enhancement role in the occurrence and development of NSCLC. Moreover, it may be a bad indicator to NSCLC patients’ prognosis.