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褐飞虱Nilaparvata lugens(Stl)是危害水稻的主要虫害之一,发掘和利用新的抗褐飞虱基因培育抗性品种是目前防治褐飞虱最经济有效的方法之一。抗褐飞虱基因来自药用野生稻的抗虫品种B5,对褐飞虱生物型1和2具有高度抗性,B5携带的抗性基因Bph14被定位在第3染色体上。本研究以B5-10为抗源,以优良杂交稻亲本扬稻6号为受体亲本,通过复交和回交,利用与Bph14紧密连锁的分子标记MRG2329在后代中进行分子标记辅助选择,通过苗期分子标记检测和成株期农艺性状选择,最后育成恢复系R476和杂交组合广两优476。采用苗期群体鉴定技术对R476和广两优476的褐飞虱抗性进行了鉴定,R476和广两优476的抗性水平分别为中抗和中感。广两优476在稻飞虱发生较重的稻田进行试种示范,与对照品种扬两优6号和两优培九相比,广两优476对稻飞虱表现出明显的抗性。研究结果表明在育种过程中利用分子标记辅助选择Bph14基因是培育抗褐飞虱水稻品种的有效途径之一。
Nilaparvata lugens (Stl) is one of the major pests that endanger rice. It is one of the most economical and effective methods to control brown planthopper at present. B5, a BPH resistant gene from medicinal wild rice, is highly resistant to BPH biotypes 1 and 2, and Bph14, a B5-resistant gene, is located on chromosome 3. In this study, B5-10 was used as the resistant source, and Yangdao 6, an excellent hybrid rice parent, was used as the parent of the recipient. Through the reciprocal mating and backcrossing, the molecular marker MRG2329, closely linked to Bph14, Molecular marker detection and selection of agronomic traits at the adult stage. Finally, restorer line R476 and hybrid combination Guanglou 476 were established. Resistance to N. lugens of R476 and Guanglougyou476 was identified by seedling population identification technique. The resistance levels of R476 and Guangliangyou 476 were medium resistance and moderate susceptibility, respectively. Guangliangyou 476 was tested for demonstration in rice fields where heavy rice planthopper occurred. Compared with Liangyoupeijiu, the two cultivars, Guangliangyou 476 showed obvious resistance to planthopper. The results show that the use of molecular markers assisted selection Bph14 gene in breeding process is one of the effective ways to cultivate rice varieties resistant to BPH.