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目的：测定霍山石斛茎中抗氧化酶等活性物质，探讨其合理的采收标准。方法：采用７５１ＧＷ紫外－可见分光光度计测定霍山三种石斛茎中ＳＯＤ，ＣＡＴ，ＰＯＤ酶活性；可溶性蛋白质含量及ＭＤＡ的含量。结果：霍山石斛三年茎ＳＯＤ，ＣＡＴ，ＰＯＤ酶活性及可溶性蛋白质含量最高，抗氧化能力强；而铁皮石斛、铜皮石斛二年茎抗氧化能力最强，三年茎已衰老。结论：霍山石斛可采用“存三去四”的采收标准；而铁皮石斛、铜皮石斛则采用“存二去三”的采收标准。
Objective: To determine the antioxidant enzymes and other active substances in the stem of Dendrobium huoshanense and to explore its reasonable harvesting standards. Methods: The activities of SOD, CAT and POD enzymes, soluble protein content and MDA content in the stems of three species of Dendrobium huoshanense were determined using a 751 GW UV-visible spectrophotometer. Results: The stem SOD, CAT, POD activity and soluble protein content of stems were highest in the three years, and the antioxidant capacity was strong. Dendrobium candidum and Dendrobium candidum had the strongest anti-oxidation ability for three years, and the stems had been aged for three years. Conclusion: Dendrobium huoshanense can adopt the standard of “retaining three to four”; while Dendrobium candidum and copper skin canopy use the criterion of “storing two to three”.