论文部分内容阅读
用ESR捕捉技术检测大鼠AM释放的活性氧自由基的性质表明:1.PMA和BCG均能刺激AM产生较强的OH·;能刺激人末稍血白细胞释放活性氧自由基的ConA和顺铂在本实验条件下未能使AM产生活性氧自由基信号。2.经膜活性剂PMA刺激的AM所释放活性氧自由基的高峰在刺激后2min,而经颗粒性物质BCG刺激,AM释放自由基的高峰时间明显后移。3.测试体系中的AM数过多或过少都不适合捕捉ESR信号。在本实验条件下,捕捉到最高自由基信号的AM终浓度为5×10~7AM/ml。4.测试体系中存在DETAPAC或EDTA,可使捕捉到的自由基信号明显增强。
The ESR capture technique was used to detect the release of active oxygen free radicals in AM. The results showed that: 1. Both PMA and BCG could stimulate AM to produce stronger OH ·; ConA, which stimulated the release of reactive oxygen species from human peripheral blood cells Platinum in the experimental conditions failed to make AM produce reactive oxygen free radical signal. The peak of active oxygen free radical released by PMA stimulated by membrane active agent was 2min after stimulation, while the peak time of free radical released by AM significantly shifted after stimulated by BCG. 3. Too much or too little AM in the test system is not suitable for capturing ESR signals. Under the experimental conditions, the final concentration of AM which captured the highest free radical signal was 5 × 10 ~ 7 AM / ml. 4. The presence of DETAPAC or EDTA in the test system significantly enhances the captured free radical signal.