论文部分内容阅读
目的观察异硫氰酸苯己酯(PHI)体外抑制骨髓瘤细胞增殖的机制。方法 MTT检测PHI对骨髓瘤细胞株RPMI8226增殖的影响;流式细胞术检测药物处理后细胞周期的变化;实时荧光定量PCR检测PHI对Jag2、发状相关增强子基因(Hes1)及人第10号染色体缺失的磷酸酶及张力蛋白同源的基因(PTEN)表达的影响。结果 PHI可抑制RPMI8226细胞增殖,使细胞滞留于G0/G1期。剂量较大的2个实验组较对照组,Jag2表达量的减少与PTEN表达量的增加具有统计学意义(P<0.05);各实验组较对照组Hes1表达量减少均具统计学意义(P<0.05)。结论 PHI可通过靶向抑制Notch信号,上调抑癌基因PTEN,抑制骨髓瘤细胞株的体外增殖。
Objective To investigate the mechanism of PHI inhibiting the proliferation of myeloma cells in vitro. Methods The proliferation of myeloma cell line RPMI8226 was detected by MTT assay. The cell cycle was detected by flow cytometry. The expression of Jag2, hairpin-related enhancer gene (Hes1) and human No. 10 Chromosomal deletion phosphatase and tensin homologue of the gene (PTEN) expression. Results PHI could inhibit the proliferation of RPMI8226 cells and keep the cells in the G0 / G1 phase. Compared with the control group, the decrease of Jag2 expression and the increase of PTEN expression were significant (P <0.05). The decrease of Hes1 expression in each experimental group was statistically significant (P <0.05). Conclusion PHI can inhibit the proliferation of myeloma cell lines by targeting Notch signaling and up-regulating tumor suppressor gene PTEN.