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目的构建表达我国钩体强毒赖型０１７株外膜蛋白的重组质粒。方法用ＰＣＲ方法扩增并回收０１７株钩体外膜蛋白基因片段ＯｍｐＬ１，将其与质粒ｐＢＫ－ＣＭＶ重组，通过菌落颜色和酶谱分析筛选出重组质粒。然后从该重组质粒切下ＯｍｐＬ１基因片段，重新克隆至质粒ｐＢＶ２２０中，酶谱分析和ＤＮＡ杂交鉴定出正向重组质粒。将含有正向重组质粒的宿主菌诱导表达生长后，提取全菌总蛋白进行ＳＤＳ－ＰＡＧＥ分析。结果筛选出４个与ｐＢＶ２２０正向重组的质粒，其中一株带重组质粒ｐＢＬＭ１的菌株表达了一相对分子质量为３３．５×１０３的新蛋白。结论重组质粒ｐＢＬＭ１的构建和表达成功说明扩增得的ＯｍｐＬ１基因具有完整的阅读框架，并使简便获得大量天然的０１７株钩体ＯｍｐＬ１蛋白成为可能，为新型钩体疫苗的研究奠定了基础
Objective To construct a recombinant plasmid expressing 017 outer membrane protein of virulent strain of Leptospira. Methods 017 strains of OmpL1 gene were amplified by PCR and recombined with plasmid pBK-CMV. The recombinant plasmid was screened by colony color and zymogram. The OmpL1 gene fragment was excised from the recombinant plasmid and cloned into the plasmid pBV220 again. The positive recombinant plasmid was identified by zymography and DNA hybridization. After the host bacterium containing the positive recombinant plasmid was induced to grow, the total bacterial total protein was extracted for SDS-PAGE analysis. Results Four plasmids were obtained by direct positive recombination with pBV220. Among them, one strain with recombinant plasmid pBLM1 expressed a new protein with relative molecular mass of 33.5 × 103. Conclusion The construction and expression of the recombinant plasmid pBLM1 successfully demonstrated that the amplified OmpL1 gene has a complete reading frame and made it easy to obtain a large number of natural 017 clades of OmpL1 protein, which laid the foundation for the study of new clade vaccine