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目的测定石黄抗菌片中黄芩苷的含量。方法采用RP-HPLC法测定黄芩苷的含量。色谱柱:Phenomenex C_(18)柱,250mm×4.60mm,5μm;流动相:甲醇-水-磷酸(47:53:0.2);检测波长:280nm;柱温:35℃;进样量:10μl。结果黄芩苷回归方程为Y=42627X-19658,r=0.9999,线性范围为9.04μg/ml～90.4μg/ml,平均回收率为99.3%。结论方法简便,快速,准确,为完善和提高该制剂的质量标准提供了依据。
Objective To determine the content of baicalin in Shihuang Antibacterial Tablets. Methods The content of baicalin was determined by RP-HPLC. Column: Phenomenex C 18 column, 250 mm x 4.60 mm, 5 μm; mobile phase: methanol-water-phosphoric acid (47:53:0.2); detection wavelength: 280 nm; column temperature: 35°C; injection volume: 10 μl. Results The baicalin regression equation was Y=42627X-19658, r=0.9999, the linear range was 9.04μg/ml to 90.4μg/ml, and the average recovery was 99.3%. Conclusion The method is simple, rapid, accurate and provides basis for improving and improving the quality standard of the preparation.