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目的观察新疆红树莓果实提取物(EER)对自发性高血压大鼠(SHR)血压的影响,并从抗氧化角度探讨其作用机制,以便为树莓的心血管保护作用提供科学依据。方法将16周龄雄性SHR32只随机分成4组:SHR对照组(SHR组,n=8)、EER低剂量组[EERL组,100mg/(kg·d),n=8]、EER高剂量组[EERH组,200mg/(kg·d),n=8]和卡托普利组[12.5mg/(kg·d),n=8],另选8只WKY大鼠作为正常血压对照组(WKY组,n=8)。采用无创尾压法测量血压,干预5周后采集血液及组织标本,检测大鼠血液一氧化氮、超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)和丙二醛含量;采用RT-PCR技术,检测大鼠主动脉一氧化氮合成酶(eNOS)mRNA,p22phox mRNA的表达。结果EER能明显降低SHR血压[SHR组(187.7±7.5)比EERL组(163.0±5.5)、EERH组(158.4±6.9)mmHg,P<0.01]。显著提高SHR血清一氧化氮水平[SHR组(13.3±5.8)比EERL组(24.9±8.7)μmol/L,P<0.01];SOD活性[SHR组(273.0±13.3)比EERL组(323.2±14.8)、EERH组(316.9±11.6)U/mL,P<0.01]以及总抗氧化能力[SHR组(7.8±1.2)比EERH组(9.8±1.6)U/mL,P<0.01];降低血清丙二醛水平[SHR组(6.8±0.8)比EERH组(6.0±0.8)μmol/L,P<0.05]。EER提高SHR主动脉一氧化氮合成酶(eNOS)mRNA表达水平[SHR组(0.88±0.24)比EERL组(1.69±0.14)、EERH组(1.49±0.24),P<0.01];降低p22phox mRNA表达水平[SHR组(1.48±0.22)比EERL组(0.66±0.07)、EERH组(0.52±0.22),P<0.01]。单因素相关分析表明,SHR血压与血清一氧化氮、SOD、T-AOC、主动脉eNOS mRNA表达呈负相关,而与血清丙二醛和主动脉p22phox mRNA水平呈正相关。结论树莓提取物能轻中度降低SHR血压,其作用可能与其抗氧化作用有关,通过提高机体SOD、T-AOC、一氧化氮以及eNOS表达水平,降低p22phox表达水平,从而减少活性氧生成,改善内皮功能有关。
Objective To observe the effect of Xinjiang Red Raspberry Fruit Extract (EER) on the blood pressure of spontaneously hypertensive rats (SHR) and to explore its mechanism from the perspective of antioxidation in order to provide a scientific basis for the cardiovascular protection of raspberry. Methods SHR control group (SHR group, n = 8), EER low dose group [EERL group, 100mg / (kg · d), n = 8] (EERH group, 200mg / (kg · d), n = 8] and captopril group [12.5mg / (kg · d), n = 8], and another 8 WKY rats were used as normal blood pressure control group WKY group, n = 8). Blood pressure was measured by noninvasive tail pressure method. Blood and tissue samples were collected 5 weeks after intervention. Blood levels of nitric oxide, superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and malondialdehyde RT-PCR was used to detect the expression of eNOS mRNA and p22phox mRNA in rat aorta. Results EER significantly decreased SHR blood pressure (187.7 ± 7.5 in SHR group, 163.0 ± 5.5 in EERL group and 158.4 ± 6.9 mmHg in EERH group, P <0.01). (13.3 ± 5.8) in SHR group (24.9 ± 8.7) μmol / L, P <0.01]. The activity of SOD in SHR group (273.0 ± 13.3) was significantly higher than that in EERL group (323.2 ± 14.8) ), EERH group (316.9 ± 11.6 U / mL, P <0.01) and total antioxidant capacity (7.8 ± 1.2 in SHR group, 9.8 ± 1.6 U / mL in EERH group, P <0.01) Dialdehyde levels [6.8 ± 0.8 in SHR group (6.0 ± 0.8) μmol / L, P <0.05] were higher than those in EERH group. EER increased the expression of eNOS mRNA in SHR group (0.88 ± 0.24 vs 1.69 ± 0.14, 1.49 ± 0.24, P <0.01) in EERH group The level of [SHR group (1.48 ± 0.22) vs EERL group (0.66 ± 0.07), EERH group (0.52 ± 0.22), P <0.01]. Univariate correlation analysis showed that there was a negative correlation between SHR blood pressure and serum nitric oxide, SOD, T-AOC and aorta eNOS mRNA expression, but positively correlated with serum malondialdehyde and aorta p22phox mRNA level. Conclusion Raspberry extract can moderately reduce the blood pressure of SHR, which may be related to its antioxidative effect. It can reduce the expression of reactive oxygen species (ROS) by increasing the expression of SOD, T-AOC, nitric oxide and eNOS and decreasing the expression of p22phox, Improve endothelial function.