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本研究所用标本取自25例孕妇在硬膜外麻醉下未用催产素前行子宫下段横切口剖腹产子宫峡部小肌束。孕妇孕期均未接受过催产素、硫酸镁、抗分娩因子或其他药物治疗。标本贮于冷藏的Krebs缓冲液,制成宽0.05～0.07mm、厚0.02～0.03mm、长0.5mm小肌肉块后,放在单独的蔗糖——脱漏仪内分析,以张力转换器记录压力。在<0.1mg静息张力下,钾和催产素可以使肌肉产生最大的收缩力,并可消除其他成份的干扰和在一定张力下细胞膜自发性兴奋的影响。Krebs缓冲液含(mmol/L):Na~+137.4、K~+5.9、Mg~(++)0.3、Ca~(++)2.5、Cl~-134、碳酸氢盐15.5、磷酸根1.2、糖11.5,并吹入97%氧及3%二氧化碳。
The specimens used in this study were obtained from 25 pregnant women undergoing epidural anesthesia without oxytocin before the uterine segment of the lower uterine incision cesarean section isthmus small muscle bundle. Pregnant women have not received during pregnancy oxytocin, magnesium sulfate, anti-labor factors or other drug treatment. Specimens were stored in refrigerated Krebs buffer and made into 0.05-0.07 mm wide, 0.02-0.03 mm thick, 0.5 mm long muscle pieces and analyzed in a separate sucrose-lysimeter to record the pressure using a tension transducer. At <0.1 mg resting tension, potassium and oxytocin produce maximum contractility in the muscles and eliminate the effects of other components and the spontaneous excitability of the cell membrane under a certain tension. Krebs buffer contains (NaCI) + 137.4, K + 5.9, Mg ++ 0.3, Ca ++ ++, Cl- 134, bicarbonate 15.5, phosphate 1.2, Sugar 11.5, and blown into 97% oxygen and 3% carbon dioxide.