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目的:探讨左旋咪唑(LMS)对大鼠实验性自身免疫性脑脊髓炎(EAE)脊髓IL-10、IL-12/23P40mRNA表达的影响。方法:用GPSCH和CFA混匀乳剂免疫Wistar大鼠制作EAE模型,分别在免疫前(LMS1组)、免疫同时(LMS2组)、免疫后(LMS3组)给LMS10mg·kg-1。比较各组行为学变化,用RT-PCR测脊髓IL-10和IL-12/23P40mRNA的表达。结果:LMS1组和LMS2组行为学表现加重(P<0.01,P<0.05)。LMS3组出现EAE复发。与EAE组比,LMS1组、LMS2组和LMS3组脊髓IL-12/23P40mRNA表达不同程度升高,IL-10mRNA表达不同程度降低。结论:LMS可升高脊髓IL-12/23P40mRNA表达,降低IL-10mRNA表达,提示LMS对EAE的促发作用可能与Th1/Th2失衡有关。
Objective: To investigate the effect of levamisole (IL-10) on the expression of IL-10 and IL-12 / 23P40 mRNA in the spinal cord of experimental autoimmune encephalomyelitis (EAE) rats. Methods: Wistar rats were immunized with GPSCH and CFA to make EAE model. The rats were killed before LMS1 immunization (LMS1 group), immunized with LMS2 group (LMS3 group), and injected with LMS3 (10 mg · kg-1). The behavioral changes of each group were compared. The expression of IL-10 and IL-12 / 23P40 mRNA in spinal cord was detected by RT-PCR. Results: The behavior of LMS1 group and LMS2 group increased significantly (P <0.01, P <0.05). EAE recurrence occurred in LMS3 group. Compared with EAE group, the expression of IL-12 / 23P40 mRNA in LMS1 group, LMS2 group and LMS3 group increased to different degrees and the expression of IL-10 mRNA decreased to some extent. CONCLUSION: LMS can increase the expression of IL-12 / 23P40mRNA in the spinal cord and decrease the expression of IL-10mRNA, suggesting that LMS may play an important role in the pathogenesis of EAE and the imbalance of Th1 / Th2.