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流式细胞术(FCM)测定68例胃癌患者术前及41例对照组外周静脉血 T 淋巴细胞及其亚群,并观察了胃癌患者术后(第7～10天)CD_4/CD_B的变化。与对照组相比,进展期胃癌 CD_3~+、CD_4~+细胞数量明显降低,CD_5~+细胞轻度升高,导致 CD_4/CD_6比值显著降低,而且 CD_4/CD_比值的这种改变与病期、肿瘤的浸润深度、淋巴结转移、肿瘤大小及生长方式相关,而与年龄、性别、肿瘤的位置及组织学分类无关。胃癌术后 CD_4/CD_比值在Ⅲ_b、Ⅳ期患者有进一步降低的趋势。提示,进展期胃癌免疫功能低下与 CD~+细胞数量降低或功能缺陷相关,而且随疾病的发展进一步加深。CD_4/CD_比值变化与胃癌生物学特征相关,因而 T 淋巴细胞亚群是一预后因素;病期、淋巴结转移、切端癌残留等因素影响术后 CD_/CD_比值恢复。结果表明,胃癌患者免疫状态是影响胃癌预后的一个重要细胞生物学因素。
Flow cytometry (FCM) was used to measure T lymphocytes and their subgroups in peripheral blood of 68 gastric cancer patients and 41 control groups before operation. The changes of CD_4/CD_B after gastric cancer patients (7th to 10th days) were observed. Compared with the control group, the number of CD_3~+ and CD_4~+ cells in advanced gastric cancer was significantly lower, and CD_5~+ cells were slightly elevated, resulting in a significant decrease in the ratio of CD_4/CD_6, and this change in CD_4/CD_ ratio was associated with disease. Period, tumor invasion depth, lymph node metastasis, tumor size and growth pattern were related to age, sex, tumor location and histological classification. The ratio of CD_4/CD_ ratio in gastric cancer after surgery was further decreased in patients with III_b and IV stages. It is suggested that the immune function of advanced gastric cancer is related to the decrease of the number of CD~+ cells or functional defects, and it will further deepen with the development of the disease. The change of CD4/CD ratio is related to the biological characteristics of gastric cancer. Therefore, T lymphocyte subsets are a prognostic factor. The factors such as the disease stage, lymph node metastasis, and residual cancer of the cut end affect the recovery of CD_/CD_ ratio. The results showed that the immune status of gastric cancer patients is an important cellular biological factor that influences the prognosis of gastric cancer.