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目的探讨组织中TNF-α含量及mRNA表达变化在老年MODS中的作用。方法老年组(20月龄)和青年组(3月龄)SD大鼠各40只,建立油酸/脂多糖(OA/LPS)二次致伤老年MODS和青年MODS模型。观察对照组及伤后2、6、24h重要器官(肺、心、肝、肾)病理及功能的变化,ELISA法检测肺、心、肝和肾组织中TNF-α含量,RT-PCR法检测不同组织中TNF-αmRNA表达。结果OA/LPS二次致伤MODS模型,肺脏是损害出现最早也是最重的器官,致伤后2h青年组和老年组PaO2即显著下降至最低值(P<0.01),伤后6h,心、肝、肾生化指标达峰值(P<0.05~0.01),在相同时相点老年鼠脏器损害重于青年鼠(P<0.05~0.01)。致伤2h后肺、心、肝和肾组织中TNF-αmRNA表达及蛋白含量均显著升高(P<0.05~0.01),且持续高于正常对照组(P<0.05~0.01),以肺组织中升高幅度最大,老年组显著高于同时相点青年组(P<0.05)。结论OA/LPS致伤早期老年鼠肺组织中TNF-αmRNA表达及蛋白含量迅速升高,可能是老年MODS肺损伤出现最早且重于青年MODS的机制之一。
Objective To investigate the role of TNF-α content and mRNA expression in aging MODS. Methods A total of 40 adult Sprague-Dawley rats aged 20 months and 3 years old were divided into two groups according to OA / LPS. The pathological changes and function of vital organs (lung, heart, liver and kidney) at 2, 6 and 24 hours after injury were observed. The contents of TNF-αin lung, heart, liver and kidney were detected by ELISA. TNF-α mRNA expression in different tissues. Results The MODS model was induced by OA / LPS. The lungs were the earliest and heaviest organ damage. After 2 hours of injury, PaO2 in young group and elderly group decreased significantly to the lowest (P <0.01) Liver and kidney biochemical indicators reached the peak (P <0.05 ~ 0.01), at the same time point organ damage in older mice than in young rats (P <0.05 ~ 0.01). After 2 hours of injury, the expression of TNF-α mRNA and protein in lung, heart, liver and kidney were significantly increased (P <0.05-0.01), and were consistently higher than those in normal control group (P <0.05-0.01) In the elderly group was significantly higher than the same phase point youth group (P <0.05). Conclusions The expression of TNF-αmRNA and the protein content in the lungs of OA rats with OA / LPS-induced early injury are rapidly increased, which may be one of the mechanisms that the earliest and heavier MODS of lung injury in aged MODS occurs.