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目的 检测金黄色葡萄球菌 (金葡菌 )的耐药表型 ,指导临床合理用药。方法 对临床分离的金葡菌用琼脂筛选法区分耐甲氧西林金葡菌 (MRSA)和甲氧西林敏感金葡菌 (MSSA) ;用碘试管法和酸试管法测定 β -内酰胺酶 ,用K -B法进行药敏试验。结果 6 6株金葡菌中 ,MRSA占 45 .5 % ,产两种 β -内酰胺酶的百分率分别是84.8% (青霉素酶 )和 5 7.6 % (头孢菌素酶 ) ,MRSA和MSSA产酶率无明显差异。除万古霉素外 ,MRSA对 11种抗生素的耐药率介于 33.3%～ 10 0 % ,而多数MSSA对青霉素和红霉素耐药 ,对其它 9种抗生素的耐药率明显低于MRSA。结论 实验室应重视鉴别MRSA和MSSA ,加强金葡菌的耐药性监测。
Objective To detect the resistant phenotype of Staphylococcus aureus (Staphylococcus aureus), and to guide clinical rational drug use. Methods Staphylococcus aureus isolated from clinical isolates were identified by MRSA and methicillin - resistant Staphylococcus aureus (MSSA) by agar screening method. Β - lactamase, K-B method for drug susceptibility testing. Results Among the 6 strains of Staphylococcus aureus, MRSA accounted for 45.5%. The percentages of producing two kinds of β - lactamases were 84.8% (penicillinase) and 5 7.6% (cephalosporins), MRSA and MSSA No significant difference in rates. In addition to vancomycin, the resistance rate of MRSA to 11 antibiotics ranged from 33.3% to 100%, while most of the MSSA strains were resistant to penicillin and erythromycin. The resistance rate to the other 9 antibiotics was significantly lower than that of MRSA. Conclusion Laboratory should pay attention to the identification of MRSA and MSSA, strengthen the drug resistance of Staphylococcus aureus surveillance.