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目的:对比泽泻炮制前后对大鼠胃泌素含量以及十二指肠Na+-K+-ATP酶活性的影响,探讨泽泻炮制前后健脾作用的变化。方法:取50只雄性SD大鼠随机分为空白组、泽泻生品低剂量组、泽泻生品高剂量组、泽泻麸制品低剂量组、泽泻麸制品高剂量组,空白组ig给予生理盐水,各低、高剂量组每次ig给予对应药物2.0,6.0 g·kg-1。每天ig给药2次,7 d后测定大鼠血清胃泌素水平及十二指肠中Na+-K+-ATP酶活性。采用离体器官实验法,以大鼠离体十二指肠平滑肌的收缩幅度、相对张力及收缩频率为指标,观察在克氏液中泽泻炮制前后提取液对大鼠离体十二指肠平滑肌的影响。结果:泽泻麸制品高剂量组血清胃泌素含量为(3.77±0.49)ng·L-1,明显高于相同剂量生品组(3.24±0.13)ng·L-1(P<0.05);麸制品高剂量组Na+-K+-ATP酶含量为(13.31±1.99)pmol·g-1,明显高于相同剂量生品组(11.77±1.37)pmol·g-1(P<0.05);生品和麸制品在高浓度时,对大鼠离体十二指肠收缩振幅及相对张力都有增强(P<0.05)。结论:泽泻能增加大鼠血清胃泌素含量、提高十二指肠Na+-K+-ATP酶活性,以及大鼠离体十二指肠肠管的运动功能,且呈剂量依赖关系,炮制后作用增强。
Objective: To compare the effect of Alisma orientalis preparation on gastrin content and Na + -K + -ATPase activity in rats before and after the preparation of Alisma orientalis before and after the preparation of Alisma orientalis spleen. Methods: Fifty male Sprague-Dawley rats were randomly divided into three groups: blank group, low-dose Alisma orientalis group, high-dose Alisma orientalis group, low-dose Alisma orientalis bran group and high- Physiological saline was given to each of the low and high dose groups at a dose of 2.0 and 6.0 g · kg -1, respectively. Administration of ig daily 2 times, 7 days after the determination of serum gastrin levels and duodenal Na + -K + -ATPase activity. The isolated organ test was used to measure the contractile amplitude, relative tension and contraction frequency of isolated rat duodenal smooth muscle. The effects of the extract before and after Alisma orientalis preparation on the isolated rat duodenum Smooth muscle effects. Results: Serum gastrin level of high dose group of Zexie bran product was (3.77 ± 0.49) ng · L-1, which was significantly higher than that of the same dose raw product group (3.24 ± 0.13) ng · L-1 (P <0.05). The content of Na + -K + -ATPase in the high-dose bran product was (13.31 ± 1.99) pmol · g-1, which was significantly higher than that of the same dose raw product group (11.77 ± 1.37) pmol · g-1 And bran products in high concentrations, the rat isolated duodenal contraction amplitude and relative tension increased (P <0.05). Conclusion: Alisma can increase serum content of gastrin, increase duodenal Na + -K + -ATPase activity, as well as isolated rat duodenum intestine motor function, and in a dose-dependent manner, the role of post-processing Enhanced.