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目的:探讨IgG、IgA、IgM、C3、C4抗核抗体(ANAS)、可提取的核抗原(ENA)和抗双链脱氧核糖核酸抗体(ds-DNA)联合检测对于诊断系统性红斑狼疮(SLE)的价值。方法:IgG、IgA、IgM、C3、C4的检测采用散射速率免疫比浊法,ANAS采用间接荧光法,抗ds-DNA采用斑点免疫金胶体渗滤法、ENA采用免疫印记法,分别对56例活动期SLE患者、71例非活动期SLE患者的血清与50例健康对照组进行对比分析。结果:SLE活动组患者IgG、IgA、IgM指标均显著高于对照组,C3、C4指标显著低于对照组,P<0.01。SLE非活动组IgG与IgA指标显著高于对照组P<0.05,其它各项检验指标与对照组相比无统计学差异性,P>0.05。结论:免疫学检验联合检测对于SLE的临床诊断以及病情进展判断均具有十分重要的意义。
OBJECTIVE: To investigate the diagnostic value of combined detection of IgG, IgA, IgM, C3, C4 ANAS, ENA and ds-DNA in the diagnosis of systemic lupus erythematosus )the value of. Methods: IgG, IgA, IgM, C3 and C4 were detected by immunosorbent assay with indirect immunofluorescence assay, ANAS indirect immunofluorescence assay, anti-ds-DNA immunofluorescence assay and ENA immunostaining method. Active SLE patients, 71 patients with inactive SLE sera and 50 healthy controls were compared. Results: The levels of IgG, IgA and IgM in SLE group were significantly higher than those in control group, and the indexes of C3 and C4 were significantly lower than those in control group (P <0.01). The indexes of IgG and IgA in inactive SLE group were significantly higher than those in control group (P <0.05). There was no significant difference between the other indexes in test group and control group (P> 0.05). Conclusion: The combination of immunological tests for the clinical diagnosis of SLE and determine the progress of the disease are of great significance.