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目的:建立同时测定满山红药材中金丝桃苷、槲皮素、山柰酚和杜鹃素含量的高效液相色谱方法。方法:采用Agilent Zorbax SBC18色谱柱(4.6mm×150mm,5μm);流动相为甲醇(A)-0.2%磷酸水溶液(B),梯度洗脱(0～20min,35%A→60%A;20～40min,60%A→70%A),流速0.8mL.min-1;检测波长为360nm(0～29min,测定金丝桃苷、槲皮素和山柰酚)和299nm(29～40min,测定杜鹃素);柱温25℃。结果:金丝桃苷、槲皮素、山柰酚和杜鹃素线性范围分别为6.40～160.0μg.mL-1(r=0.9995),3.33～83.33μg.mL-1(r=0.9993),0.80～20.0μg.mL-1(r=0.9998),2.67～66.67μg.mL-1(r=0.9996);金丝桃苷、槲皮素、山柰酚和杜鹃素回收率(n=3)分别为96.5%～99.2%(RSD为1.2%～1.6%),96.6%～98.9%(RSD为0.9%～2.8%),96.4%～101.7%(RSD为2.0%～2.9%),95.9%～100.4%(RSD为0.4%～2.2%)。结论:该方法简便快速,结果准确可靠,可用于中药满山红的质量控制。
Objective: To establish a HPLC method for the simultaneous determination of hyperoside, quercetin, kaempferol and farrellin in Mangshanhong medicinal materials. Methods: Agilent Zorbax SBC18 column (4.6mm × 150mm, 5μm) was used. The mobile phase consisted of methanol (A) -0.2% phosphoric acid solution (B), gradient elution ~ 40min, 60% A → 70% A) at a flow rate of 0.8mL.min-1. The detection wavelength was 360nm (0 ~ 29min, measured hyperoside, quercetin and kaempferol) and 299nm Determination of farrerol); column temperature 25 ℃. Results: The linear ranges of hyperoside, quercetin, kaempferol and farrell were 6.40-160.0μg.mL-1 (r = 0.9995), 3.33-83.33μg.mL-1 (r = 0.9993) (R = 0.9998) and 2.67 ~ 66.67μg.mL-1 (r = 0.9996) respectively. The recoveries of hyperin, quercetin, kaempferol and farrell (n = 3) Were 96.5% -99.2% (RSD 1.2% -1.6%), 96.6% -98.9% (RSD 0.9% -2.8%), 96.4% -101.7% (RSD 2.0% -2.9%), 95.9% -100.4 % (RSD 0.4% ~ 2.2%). Conclusion: The method is simple and rapid, the results are accurate and reliable, and can be used for the quality control of traditional Chinese medicines.