论文部分内容阅读
目的:探讨LIF对人胚神经干细胞(NSCs)增殖和分化的影响。方法:从孕24周流产胎儿脑组织中分离NSCs,并分4组培养。对照组用基础培养液,第1,2,3实验组分别于基础培养液中加入20μg/L EGF和B27,20μg/L LIF和B27,20μg/L LIF、20μg/L EGF及B27。观察细胞生长情况并在不同时间进行细胞计数。诱导细胞分化后,显微镜下观察。结果:培养3 d细胞开始分裂并聚集成球,后逐渐增大,培养10 d,第1,2,3实验组和对照组神经球形成数分别为(21.5±7.8),(23.8±5.4),(25.2±6.3)和(24.8±6.9),各组间差异无统计学意义。分化实验显示含LIF培养的神经干细胞分化少。结论:在体外,LIF能够促进神经干细胞的增殖并抑制神经干细胞的分化。
Objective: To investigate the effect of LIF on the proliferation and differentiation of human embryonic neural stem cells (NSCs). Methods: NSCs were isolated from aborted fetal brain tissue 24 weeks pregnant and divided into 4 groups. The control group with basal medium, the first experimental group 1,2,3 were added to the basal medium 20μg / L EGF and B27, 20μg / L LIF and B27, 20μg / L LIF, 20μg / L EGF and B27. Observed cell growth and cell counts at different times. After inducing cell differentiation, the cells were observed under a microscope. RESULTS: After 3 days of culture, the cells began to divide and aggregate into spheres, and then gradually increased. After cultured for 10 days, the numbers of neurospheres in experimental groups 1, 2 and 3 were (21.5 ± 7.8) and (23.8 ± 5.4) , (25.2 ± 6.3) and (24.8 ± 6.9) respectively. There was no significant difference among the groups. Differentiation experiments showed that LIF-cultured neural stem cells did not differentiate. Conclusion: In vitro, LIF can promote the proliferation of neural stem cells and inhibit the differentiation of neural stem cells.