论文部分内容阅读
目的:建立免疫亲和柱超高效液相色谱法测定食品中黄曲霉毒素B1,B2,G1和G2含量的方法。方法:试样经过甲醇-水提取、稀释后经过免疫亲和柱层析净化,应用超高液相色谱法检测。结果:试验结果表明:空白样品分别按照0.2μg/kg、0.8μg/kg、2.0μg/kg添加黄曲霉毒素混合标准,回收率为75.0%～90.4%,精密度<5%,黄曲霉毒素B1,B2,G1和G2的检测灵敏度分别为0.2μg/kg,0.2μg/kg,0.4μg/kg,0.2μg/kg。结论:免疫亲和柱净化超高效液相色谱法测定食品中黄曲霉毒素B1,B2,G1,G2含量,是一种简单、快速和准确的方法。
Objective: To establish an immunoaffinity column method for the determination of aflatoxins B1, B2, G1 and G2 in food by ultra performance liquid chromatography. Methods: The sample was extracted with methanol - water, diluted and purified by immunoaffinity column chromatography. The samples were detected by ultra performance liquid chromatography. Results: The test results showed that the blank samples were mixed with aflatoxins at 0.2μg / kg, 0.8μg / kg and 2.0μg / kg, the recovery was 75.0% -90.4%, the precision was less than 5%, the aflatoxin B1 The detection sensitivity of B2, G1 and G2 were 0.2μg / kg, 0.2μg / kg, 0.4μg / kg and 0.2μg / kg, respectively. Conclusion: The method of immunoaffinity column purifie ultra high performance liquid chromatography for the determination of aflatoxins B1, B2, G1 and G2 in food is a simple, rapid and accurate method.