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目的对二孩政策全面实施后准备生育二孩的丈夫精液常规参数、精子畸形率进行分析。方法将纳入标准的研究对象分为意向生育二孩组(二孩组,n=232)、试管婴儿组(试管组,n=120)、正常生育组(正常组,n=82),精液标本充分液化后严格按照《WHO人类精液检查与处理实验手册(第五版)》进行精液常规分析,采用改良巴氏染色法,严格按照Kruger标准检测研究对象精子畸形率。结果 1二孩组、试管组、正常组的年龄分别为(37.1±5.7)、(33.0±6.2)、(28.2±3.8)岁;精液量分别为(2.8±1.7)ml、(2.8±3.5)ml、(3.1±0.9)ml;精子浓度分别为(73.4±53.6)×106/ml、(45.7±49.7)×106/ml、(79.8±48.0)×106/ml;精子总活力分别为(60.9±18.8)%、(30.7±13.0)%、(71.8±14.8)%;精子前向运动率分别为(51.2±17.9)%、(23.5±10.8)%、(61.9±15.9)%;精子畸形率分别为(87.8±5.8)%、(93.6±6.1)%、(86.5±6.7)%。2三组间进行两两比较,二孩组的精子浓度、精子总活力、精子前向运动率和正常形态精子百分比方面明显优于试管组,差异有统计学意义(P<0.01),精液量均值与试管组相同,但差异有统计学意义(P<0.05);与正常组相比,二孩组在精液量、精子总活力、精子前向运动率方面低于正常组,差异有统计学意义(P<0.01),但精子浓度、精子总畸形率方面差异无统计学意义(P>0.05);正常组在精液量、精子浓度、精子总活力、前向运动率、精子形态方面明显优于试管组,差异有统计学意义(P<0.01)。结论准备生育二孩的男性精液质量较正常生育者差异不明显,但要明显优于进行试管婴儿的男性。
Objective To analyze the routine parameters of the sperm of her husband and the sperm deformity rate after the second child policy is fully implemented. Methods The subjects enrolled in the standard were divided into two groups: infantile second child group (n = 232), IVF group (n = 120), normal fertility group (n = 82) After full liquefaction, the sperm routine analysis was carried out in strict accordance with “WHO Human Semen Inspection and Treatment Experiment Manual (Fifth Edition)”. The sperm deformity rate was tested strictly according to the Kruger standard by modified Papanicolaou staining. Results The age of the second group, the test tube group and the normal group were (37.1 ± 5.7), (33.0 ± 6.2) and (28.2 ± 3.8) years old respectively; the semen volume was (2.8 ± 1.7) ml and (2.8 ± 3.5) ml and (3.1 ± 0.9) ml respectively. The sperm concentrations were (73.4 ± 53.6) × 106 / ml, (45.7 ± 49.7) × 106 / ml, (79.8 ± 48.0) × 106 / ml respectively. ± 18.8%, 30.7 ± 13.0% and 71.8 ± 14.8% respectively. The sperm forward movement rates were (51.2 ± 17.9)%, (23.5 ± 10.8)% and (61.9 ± 15.9)%, respectively (87.8 ± 5.8)%, (93.6 ± 6.1)% and (86.5 ± 6.7)%, respectively. There was a significant difference between the two groups (P <0.01). The sperm concentration, total sperm motility, sperm motility and the percentage of normal spermatozoa in the second group were significantly better than those in the test tube group The mean value was the same as that of the test tube group, but the difference was statistically significant (P <0.05). Compared with the normal group, the second child group was lower than the normal group in semen volume, sperm motility and forward sperm motility rate (P <0.01), but there was no significant difference in sperm concentration and total sperm deformity between the two groups (P> 0.05). In the normal group, the sperm concentration, sperm concentration, sperm motility, forward motility rate and sperm morphology were significantly superior In test tube group, the difference was statistically significant (P <0.01). Conclusion There is no obvious difference in the quality of sperm between male and female who are going to give birth to two children, but they are obviously better than those who are in vitro.