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光敏核不育性受1对或2对或3对隐性主基因控制,这反应了光敏核不育特性遗传机制的复杂性。张端品等用形态标记法将农垦58S光敏核不育基因定位于第5染色体。胡学应和万邦惠用同工酶法以农垦58S/02428 F_2群体为材料,将光敏核不育基因定位于第6和11染色体;Zhang等用RFLP法以32001S/明恢63 F_2群体为材料将不育基因定位于第3和7染色体。这三种方法所得到的定位结果完全不同,综合起来,第3、5、6、7和11染色体均有光敏核不育基因的位点,由此结果可得出两种解释:1.光敏核不育性由多对基因、至少5对基因控制;2.上述定位方法均是以不育(可育)性状在F_2群体中的分离模式为依据,育性划分界线的不同将会造成分离群体中单株表现值的差异,从而影响定位结果的精确性;再者不同实验室使用的材料不一致,已知不同遗传背景和光温条件影响光敏核不育基因的表达。因此,染色体定位结果有待确证。光敏核不育基因在染色体上定位的复杂性和不一致在某种程度上影响了基因克隆和光敏核不育分子机制的研究。无论光敏核不育性的遗传机制如何复杂,上述结果
Photosensitive nuclear sterility is controlled by one or two pairs or three pairs of recessive major genes, reflecting the complexity of the genetic mechanisms of photo-sensitive nuclear infertility traits. Zhang Duanpin and other morphological markers of Nongken 58S photosensitive nuclear sterile gene located on chromosome 5. Hu Xue-ying and Wan Bang-hui used Nongken 58S / 02428 F 2 population as isozymes to map photoperiod-sensitive genic male sterility genes on chromosomes 6 and 11. Zhang et al used RFLP method to clone 32001S / Minghui 63 F 2 population as material The gene is located on chromosomes 3 and 7. The results obtained by these three methods are completely different. Taken together, chromosomes 3, 5, 6, 7 and 11 have sites of photosensitive genic male sterility genes. From these results, two explanations can be obtained: 1. Photosensitization At least 5 pairs of genes were controlled for nuclear fertility. 2. The above methods were all based on the separation pattern of sterile (fertile) traits in F_2 population, and the difference of fertility dividing line would result in segregation The difference of single plant performance in the population, thus affecting the accuracy of the positioning results; different materials used in different laboratories are inconsistent, known to have different genetic backgrounds and light and temperature conditions affect the expression of photosensitive genic male sterile gene. Therefore, chromosomal localization results to be confirmed. To some extent, the complexity and inconsistency in the mapping of photosensitive genic male sterility genes on chromosomes affect the molecular cloning and molecular mechanisms of photo-induced nuclear sterility. No matter how complex the genetic mechanism of photo-induced nuclear sterility, the above results